Erratum to: Precision of the Kalon Herpes Simplex Virus Type 2 IgG ELISA: an international inter-laboratory assessment
| Autor: | Jairam R. Lingappa, Oliver Laeyendecker, Barry Kosloff, Maina M. Cheeba, Rhoda Ashley Morrow, Mubiana Inambao, Thomas C. Quinn, Jordyn Manucci, Estelle Piwowar-Manning, Kwitaka F. Maluzi, Anelet James, Bellington Vwalika, Eshan U. Patel, Erin M. Kahle, Sinead Delany-Moretlwe, Glenda Gray |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Adult Male Herpesvirus 2 Human 030106 microbiology Zambia Enzyme-Linked Immunosorbent Assay HIV Infections Biology medicine.disease_cause Antibodies Viral Sensitivity and Specificity Microbiology lcsh:Infectious and parasitic diseases Kalon South Africa 03 medical and health sciences Sensitivity 0302 clinical medicine medicine Humans lcsh:RC109-216 030212 general & internal medicine Inter-laboratory Igg elisa Accuracy Herpes Genitalis Reproducibility of Results Herpes Precision HSV-2 Virology Reproducibility Infectious Diseases Herpes simplex virus ROC Curve Area Under Curve Immunoglobulin G Calibration Specificity Female Erratum Laboratories Research Article |
| Zdroj: | BMC Infectious Diseases BMC Infectious Diseases, Vol 17, Iss 1, Pp 1-1 (2017) |
| ISSN: | 1471-2334 |
| Popis: | Background The commercial Kalon HSV-2 IgG ELISA is currently recommended for research use in sub-Saharan Africa because of its superior accuracy compared to other serologic assays. However, there are no data on key precision parameters of Kalon such as inter-operator variation, repeatability, and reproducibility, thus contributing to a barrier for its acceptance and use in clinical trials in sub-Saharan Africa. We evaluated the analytical and field precision of the Kalon HSV-2 IgG ELISA. Methods A total of 600 HIV-infected and uninfected serum samples from South Africa and Zambia, previously tested by the gold standard University of Washington HSV western blot (UW-WB), were tested using Kalon by two technologists in an United States reference laboratory. Aliquots of 183 samples were retested using Kalon by an on-site technologist in a South African laboratory and a Zambian laboratory. Results Intra-assay variation was below 10 %. Intra-assay, intra-laboratory, and inter-laboratory correlation and agreement were significantly high (p |
| Databáze: | OpenAIRE |
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