Monitoring of inflammation using novel biosensor mouse model reveals tissue- and sex-specific responses to Western diet
| Autor: | Sarah Talley, Raiza Bonomo, Chaitanya Gavini, Jomana Hatahet, Emily Gornick, Tyler Cook, Byeong Jae Chun, Pete Kekenes-Huskey, Gregory Aubert, Edward Campbell, Virginie Mansuy-Aubert |
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| Rok vydání: | 2021 |
| Předmět: |
Inflammation
Male Inflammasomes Neuroscience (miscellaneous) Medicine (miscellaneous) Mice Obese Biosensing Techniques Diet High-Fat General Biochemistry Genetics and Molecular Biology Mice Inbred C57BL Butyrates Disease Models Animal Mice Immunology and Microbiology (miscellaneous) Diet Western Caspases Animals Female Obesity |
| Zdroj: | Disease modelsmechanisms. 15(6) |
| ISSN: | 1754-8411 |
| Popis: | Obesity is an epidemic, and it is characterized by a state of low-grade systemic inflammation. A key component of inflammation is the activation of inflammasomes, multiprotein complexes that form in response to danger signals and that lead to activation of caspase-1. Previous studies have found that a Westernized diet induces activation of inflammasomes and production of inflammatory cytokines. Gut microbiota metabolites, including the short-chain fatty acid butyrate, have received increased attention as underlying some obesogenic features, but the mechanisms of action by which butyrate influences inflammation in obesity remain unclear. We engineered a caspase-1 reporter mouse model to measure spatiotemporal dynamics of inflammation in obese mice. Concurrent with increased capsase-1 activation in vivo, we detected stronger biosensor signal in white adipose and heart tissues of obese mice ex vivo and observed that a short-term butyrate treatment affected some, but not all, of the inflammatory responses induced by Western diet. Through characterization of inflammatory responses and computational analyses, we identified tissue- and sex-specific caspase-1 activation patterns and inflammatory phenotypes in obese mice, offering new mechanistic insights underlying the dynamics of inflammation. |
| Databáze: | OpenAIRE |
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