New proteins orthologous to cerato-platanin in various Ceratocystis species and the purification and characterization of cerato-populin from Ceratocystis populicola
| Autor: | Francesca Sbrana, Aniello Scala, Alberto Santini, Federico Sebastiani, Gianni Cappugi, Nicola Luchi, B. Tiribilli, Paolo Capretti, Luigia Pazzagli, Cecilia Comparini, L. Carresi, Eleonora Pagni |
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| Rok vydání: | 2009 |
| Předmět: |
Genes
Fungal Molecular Sequence Data Biology Plant disease resistance Ceratocystis Benzoates Applied Microbiology and Biotechnology Microbiology Fungal Proteins chemistry.chemical_compound Ascomycota Glucosides Botany medicine Amino Acid Sequence Phylogeny Canker Base Sequence Sequence Homology Amino Acid Cerato-platanin Nucleic acid sequence Ceratocystis platani General Medicine Mycotoxins medicine.disease biology.organism_classification Recombinant Proteins medicine.drug_formulation_ingredient Populin chemistry Ceratocystis fimbriata Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 84:309-322 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/s00253-009-1998-4 |
| Popis: | Natural variants of cerato-platanin (CP), a pathogen associated molecular pattern (PAMP) protein produced by Ceratocystis platani (the causal agent of the plane canker stain), have been found to be produced by other four species of the genus Ceratocystis, including five clones of Ceratocystis fimbriata isolated from different hosts. All these fungal strains were known to be pathogenic to plants with considerable importance in agriculture, forestry, and as ornamental plants. The putative premature proteins were deduced on the basis of the nucleotide sequence of genes orthologous to the cp gene of C. platani; the deduced premature proteins of Ceratocystis populicola and Ceratocystis variospora reduced the total identity of all the others from 87.3% to 60.3%. Cerato-populin (Pop1), the CP-orthologous protein produced by C. populicola, was purified and characterized. Pop1 was a well-structured alpha/beta protein with a different percentage of the alpha-helix than CP, and it self-assembled in vitro in ordered aggregates. Moreover, Pop1 behaved as PAMP, since it stimulated poplar leaf tissues to activate defence responses able to reduce consistently the C. populicola growth. |
| Databáze: | OpenAIRE |
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