Protein phosphorylation in alveolar macrophages after stimulation with heavy metal-coated silica particles
| Autor: | M. Delling, M Radloff, Günther Gercken |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
inorganic chemicals
Vanadium Compounds chemistry.chemical_element Toxicology chemistry.chemical_compound Heat shock protein Macrophages Alveolar Cadmium Compounds Animals Protein phosphorylation Phosphorylation Tyrosine Polyacrylamide gel electrophoresis Heat-Shock Proteins Cadmium Chromatography Receptor Protein-Tyrosine Kinases Dust Oxides Tyrosine phosphorylation Quartz General Medicine Macrophage Activation respiratory system Silicon Dioxide Stimulation Chemical Blot Kinetics chemistry Cattle |
| Zdroj: | Toxicology Letters. :69-75 |
| ISSN: | 0378-4274 |
| Popis: | The aim of our study was to determine agonist- and time-dependent phosphorylation of distinct proteins in bovine alveolar macrophages after activation with quartz dusts and heavy metal-coated silica particles. Silica particles were coated with vanadium oxide or cadmium oxide. Separation of proteins was achieved by one-dimensional and optimized horizontal two-dimensional polyacrylamide gel electrophoresis. Protein phosphorylation was either determined by [32P] labeling and subsequent autoradiography or Western blotting using monoclonal antibodies against phosphotyrosine. One of the phosphorylated proteins was identified as the heat shock protein HSP-27. Stimulation with cadmium oxide-coated silica particles and PMA led to a significant increase of phosphorylated HSP-27. Additionally cadmium oxide-coated silica particles induced tyrosine phosphorylation of six main proteins. Stimulation with vanadium oxide-coated silica particles drastically increased the amount of tyrosine phosphorylated proteins while this effect was not observed with uncoated silica particles. |
| Databáze: | OpenAIRE |
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