DkNAC7, a novel high-CO2/hypoxia-induced NAC transcription factor, regulates persimmon fruit de-astringency
| Autor: | Qinggang Zhu, Miao-miao Wang, Donald Grierson, Xin-yue Shen, Kunsong Chen, Wajeeha Jamil, Xue-ren Yin, Rong Jin |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Gene Expression lcsh:Medicine Artificial Gene Amplification and Extension Biochemistry Polymerase Chain Reaction 01 natural sciences Gene Expression Regulation Plant Transcription (biology) Transcriptional regulation Cloning Molecular Promoter Regions Genetic Hypoxia lcsh:Science Phylogeny Plant Proteins Regulation of gene expression Multidisciplinary Chemistry Transcriptional Control Eukaryota Plants Cell biology Protein Transport Experimental Organism Systems Protein Binding Research Article Arabidopsis Thaliana DNA transcription Brassica Research and Analysis Methods Fruits 03 medical and health sciences Model Organisms Plant and Algal Models Complementary DNA DNA-binding proteins Genetics Gene Regulation Molecular Biology Techniques Molecular Biology Gene Transcription factor Genetic Association Studies Biology and life sciences cDNA library lcsh:R Organisms Proteins Cell Biology Diospyros Regulatory Proteins 030104 developmental biology Fruit lcsh:Q Pyruvate decarboxylase Transcription Factors 010606 plant biology & botany |
| Zdroj: | PLoS ONE, Vol 13, Iss 3, p e0194326 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Artificial high-CO2 atmosphere (AHCA, 95% CO2 and 1% O2) has been widely applied as a postharvest de-astringency treatment for persimmon fruit. AHCA increases expression of transcription factors, including ethylene response factors (DkERF), that target de-astringency genes. Here, the promoter of DkERF9, a previously characterized AHCA-inducible and de-astringency regulator, was utilized to screen a cDNA library by yeast one hybrid assay. A novel NAC transcription factor, named DkNAC7, was identified. Dual-luciferase assay indicated that DkNAC7 could not only trans-activate the promoter of DkERF9, but also activated the previously identified deastringency-related gene DkPDC2. Real-time PCR analysis showed that DkNAC7 was up-regulated by AHCA treatment, in concert with the removal of astringency from persimmon fruit and subcellular localization showed DkNAC7 was located in the nucleus. Thus, these results indicate that DkNAC7 is a putative transcriptional activator involved in regulating persimmon fruit deastringency by trans-activition on both DkERF9 and DkPDC2, which encodes pyruvate decarboxylase. |
| Databáze: | OpenAIRE |
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