Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers

Autor:	Valentina S. Dimitrova, Saemee Song, Alexandra Karagiaridi, Anika Marand, Heather W. Pinkett
Rok vydání:	2022
Předmět:	Polymers Detergents Lipid Bilayers Membrane Proteins Polystyrenes Lipids Article Styrenes
Zdroj:	Methods in Molecular Biology ISBN: 9781071623671 Methods Mol Biol
DOI:	10.1007/978-1-0716-2368-8_20
Popis:	The development of styrene maleic acid (SMA) and diisobutylene maleic acid (DIBMA) copolymers provides an alternative to traditional detergent extraction of integral membrane proteins. By inserting into the membrane, these polymers can extract membrane proteins along with lipids in the form of native nanodiscs made by poly(styrene co-maleic anhydride) derivatives. Unlike detergent solubilization, where membrane proteins may lose annular lipids necessary for proper folding and stability, native nanodiscs allow for proteins to reside in the natural lipid environment. In addition, polymer-based nanodiscs can be purified using common chromatography methods similar to protocols established with detergent solubilization purification. Here we describe the solubilization screening and purification of an integral membrane protein using several commercial copolymers.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af89cd7dbe7e835488693d38a0a6b18f https://doi.org/10.1007/978-1-0716-2368-8_20 Zobrazit plný text záznamu