Multilevel selection of bcrABDR- mediated bacitracin resistance in Enterococcus faecalis from chicken farms
| Autor: | Ya-Hong Liu, Hua-Qing Liang, Felipe Lira, Jia-Hong Duan, José L. Martínez, Rui-Ting Wu, Mu-Ya Chen, Xiao-Ping Liao, Jian Sun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Farms Sequence analysis 030106 microbiology Locus (genetics) Drug resistance Bacitracin Article Enterococcus faecalis Microbiology Feces 03 medical and health sciences Plasmid Drug Resistance Multiple Bacterial Pulsed-field gel electrophoresis medicine Animals Humans Symbiosis Gram-Positive Bacterial Infections Genetics Multidisciplinary biology Bird Diseases Microbiota biology.organism_classification Anti-Bacterial Agents Genetic Loci Multilocus sequence typing Chickens medicine.drug |
| Zdroj: | Scientific Reports |
| Popis: | In this study we isolated 109 Enterococcus faecalis from chicken faecal samples in 6 provinces of China to investigate the prevalence and transmission mechanism of the bacitracin resistance locus bcrABDR in E. faecalis. Thirty-seven bcrABDR-positive E. faecalis were detected with 26 di erent PFGE clusters. The MLST of 14 positive strains belonged to ST16 and we also detected three new sequence types. S1-PFGE analysis indicated that the locus was located on plasmids presenting di erent sizes, with the most prevalent size being ~50 kb (13/37). Sequence analysis revealed that 17 out of the 37 strains harbored a 5400-bp central region, in which locus bcrABDR was bracketed by two ISEnfa1 of the same orientation. Two types of bcrABDR alleles, di ering in around 10% of their sequence were found. In silico analysis showed that bcrABDR is present in a variety of bacteria including the chicken commensal Enterococcus cecorum. Our results indicate that the use of bacitracin at farms might trigger the emergence and spread of the bacitracin resistance determinant bcrABDR among human bacterial pathogens. The finding of bcrABDR in the chicken commensal E. cecorum indicates that farm animals microbiota can be an important reservoir of resistance genes with relevance for human health. |
| Databáze: | OpenAIRE |
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