Detection of multidrug-resistant Pseudomonas aeruginosa harboring bla GES-1 and bla GES-11 in Recife, Brazil
| Autor: | Laura Durão Ferreira, Valdemir Vicente da Silva Júnior, Adriane Borges Cabral, Paulo Sérgio Ramos de Araújo, Ana Catarina de Souza Lopes, Paula Regina Luna de Araújo Jácome, Maria Amélia Vieira Maciel, Lílian Rodrigues Alves |
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| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Microbiology (medical) lcsh:Arctic medicine. Tropical medicine Sequence analysis lcsh:RC955-962 030106 microbiology Drug resistance Microbial Sensitivity Tests Biology medicine.disease_cause Antimicrobial resistance beta-Lactamases Bacterial genetics law.invention Microbiology 03 medical and health sciences Intergenic region Antibiotic resistance law Drug Resistance Multiple Bacterial medicine Humans Pathogen Polymerase chain reaction Base Sequence Pseudomonas aeruginosa Sequence Analysis DNA biochemical phenomena metabolism and nutrition bacterial infections and mycoses Guiana extended spectrum beta-lactamase Anti-Bacterial Agents 030104 developmental biology Infectious Diseases Parasitology Brazil |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical, Vol 50, Iss 6, Pp 764-768 Revista da Sociedade Brasileira de Medicina Tropical, Volume: 50, Issue: 6, Pages: 764-768, Published: DEC 2017 Revista da Sociedade Brasileira de Medicina Tropical v.50 n.6 2017 Revista da Sociedade Brasileira de Medicina Tropical Sociedade Brasileira de Medicina Tropical (SBMT) instacron:SBMT |
| ISSN: | 1678-9849 |
| Popis: | INTRODUCTION: Pseudomonas aeruginosa, an important pathogen globally, presents several resistance mechanisms. This study aimed to investigate the presence of bla GES in clinical isolates of Pseudomonas aeruginosa obtained from various clinical specimens from patients admitted to three different hospitals in Recife, Brazil. The Guiana extended spectrum beta-lactamase (GES) enzymes are responsible for conferring broad spectrum resistance to beta-lactam drugs, including the carbapenems. METHODS: A total of 100 carbapenem-resistant P. aeruginosa isolates underwent polymerase chain reaction (PCR) testing to identify bla GES, bla KPC, bla SPM-1, bla IMP, and bla VIM. Additionally, PCR products positive for bla GES were sequenced. The clonal profiles of these same isolates were then determined by means of enterobacterial repetitive intergenic consensus (ERIC)-PCR analysis. RESULTS: PCR analysis revealed that four isolates harbored bla GES; DNA sequencing showed that two harbored bla GES-1 and two bla GES-11. Beta-lactamase genes bla SPM-1, bla IMP, bla VIM, and bla KPC were investigated; none of these genes was detected. Automated susceptibility testing methods (Vitek®2, bioMérieux) showed that the bla GES-1-positive isolates were only susceptible to polymyxin B. The patterns obtained with ERIC-PCR methods showed clonal relationship between the two isolates that harbored bla GES-11, whereas different clonal profiles were found in the isolates harboring bla GES-1. CONCLUSIONS: We detected the presence of bacterial isolates positive for two different variants of the enzyme GES in three different hospitals from Recife, Brazil. These enzymes have a great capacity for dissemination among Gram-negative bacteria and confer broad-spectrum resistance to beta-lactam antibiotics and to the carbapenems. |
| Databáze: | OpenAIRE |
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