Responses of Heterodera glycines and Meloidogyne incognita Infective Juveniles to Root Tissues, Root Exudates, and Root Extracts from Three Plant Species
| Autor: | Edward P. Masler, Stephen T. Rogers, Congli Wang |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Plant Exudates Soybean cyst nematode Plant Science Fractionation Plant Roots 01 natural sciences Tagetes 03 medical and health sciences Pepper Meloidogyne incognita Animals Tylenchoidea Plant Diseases biology Plant Extracts Heterodera fungi food and beverages biology.organism_classification Horticulture 030104 developmental biology Nematode Seedlings Soybeans Capsicum Agronomy and Crop Science Tagetes patula Terra incognita 010606 plant biology & botany |
| Zdroj: | Plant Disease. 102:1733-1740 |
| ISSN: | 1943-7692 0191-2917 |
| DOI: | 10.1094/pdis-09-17-1445-re |
| Popis: | The infective juvenile (J2) stage of endoparasitic plant nematodes uses plant chemical signals, released from roots, to localize and infect hosts. We examined the behaviors of soybean cyst nematode (Heterodera glycines) and root-knot nematode (Meloidogyne incognita) J2 in the presence of root signals from marigold (Tagetes patula), soybean (Glycine max), and pepper (Capsicum annuum). Signals were obtained from sources commonly used in phytoparasitic nematode chemotaxis studies: root tips, root exudates, and root extracts. Root tips from each plant species attracted M. incognita but H. glycines was attracted only to soybean. In contrast, root exudates prepared from marigold, pepper, or soybean seedlings were attractive to H. glycines but were repellent to M. incognita. Root extracts had the same effect as exudates. Fractionation of exudates by reversed-phase high-performance liquid chromatography (HPLC) (acetonitrile [CH3CN] and 0.1% trifluoroacetic acid) revealed highly polar and less polar components affecting behaviors. Fractions eluting at 12% CH3CN from all three plants attracted H. glycines and repelled M. incognita. None of the less polar HPLC fractions (>15% CH3CN) affected H. glycines but those from G. max and T. patula repelled M. incognita. Differences among exudates and effects of fractionation on behavior are discussed. |
| Databáze: | OpenAIRE |
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