Long-term therapy with recombinant human erythropoietin increases CD8+ T-cell apoptosis in haemodialysis patients
| Autor: | Piotr Trzonkowski, Andrzej Myśliwski, Jolanta Myśliwska, Bolesław Rutkowski, Łukasz Hak, Ewa Szmit, Alicja Dębska-Ślizień, Katarzyna Szymańska |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
CD4-Positive T-Lymphocytes
medicine.medical_specialty Necrosis Apoptosis CD8-Positive T-Lymphocytes Peripheral blood mononuclear cell Antigens CD Reference Values Renal Dialysis Internal medicine medicine Humans Cytotoxic T cell Lymphocyte Count Erythropoietin Cells Cultured Phytohaemagglutinin Transplantation biology business.industry CD28 DNA Middle Aged Recombinant Proteins CD4 Lymphocyte Count Endocrinology Nephrology biology.protein Kidney Failure Chronic medicine.symptom business CD8 medicine.drug |
| Zdroj: | Nephrology Dialysis Transplantation. 20:367-376 |
| ISSN: | 1460-2385 0931-0509 |
| Popis: | Background. We intended to assess the intensity of apoptosis in the CD4 + and CD8 + T-lymphocytes of haemodialysis (HD) patients on recombinant human erythropoietin (rHuEpo). Methods. The expression of Fas, tumour necrosis factor-α receptors (TNFRI and TNFRII) and the CD28 molecule on lymphocytes was evaluated in 15 HD patients before and during treatment with rHuEpo. In cultures of peripheral blood mononuclear cells (PBMCs) stimulated with rHuEpo, phytohaemagglutinin and camptothecin, our measures of apoptosis were the percentages of cells with subdiploid DNA content and of annexin V-stained cells. Results, Therapy with rHuEpo did not affect CD4 + T cells but decreased the percentage of CD8 + T cells in peripheral blood. The intensity of apoptosis in both CD4 + and CD8 + T cells at baseline was lower in HD patients than in healthy volunteers, and increased in those treated with rHuEpo. In vitro, rHuEpo did not induce apoptosis in PBMCs. The percentage of CD8 + Fas + T cells was constant, while that of CD8 + TNFRI + cells declined during follow-up. There was an increase in the percentage of CD28 + T cells, mainly in the CD8 + compartment, as early as 1 month after the introduction of rHuEpo. Conclusions. Treatment with rHupo caused a decline of CD8 + T cells in HD patients, which most probably was mediated via the TNFRI-related apoptotic pathway and was independent of Fas expression. Apoptosis in vitro was not directly influenced by rHuEpo, suggesting that the process in vivo was only initiated by rHuEpo supplementation. |
| Databáze: | OpenAIRE |
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