Data-Driven Modeling of Intracellular Auxin Fluxes Indicates a Dominant Role of the ER in Controlling Nuclear Auxin Uptake
| Autor: | Christian Fleck, Matias D. Zurbriggen, Klaus Palme, Cristina Dal Bosco, Phillip Chlap, Olaf Ronneberger, Rainer Uhl, Fugang Ren, Robert Bensch, Wilfried Weber, Alistair M. Middleton, Alexander Dovzhenko, Hartmann Harz, Ken-ichiro Hayashi, Sabrina Wend, Stefan Bergmann |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell General Biochemistry Genetics and Molecular Biology 03 medical and health sciences protoplasts single cells Auxin fluorescent aux medicine Humans Systems and Synthetic Biology heterocyclic compounds lcsh:QH301-705.5 Plant Proteins VLAG chemistry.chemical_classification Systeem en Synthetische Biologie Indoleacetic Acids Endoplasmic reticulum nucleus fungi mathematical modeling Nuclear Proteins food and beverages Master regulator Plant cell Cell biology auxin flux endoplasmic reticulum 030104 developmental biology medicine.anatomical_structure lcsh:Biology (General) chemistry auxin sensor microscopy Signal transduction auxin Nucleus Intracellular Signal Transduction |
| Zdroj: | Cell Reports, Vol 22, Iss 11, Pp 3044-3057 (2018) Cell Reports 22 (2018) 11 Cell Reports, 22(11), 3044-3057 |
| ISSN: | 2211-1247 |
| Popis: | Summary: In plants, the phytohormone auxin acts as a master regulator of developmental processes and environmental responses. The best characterized process in the auxin regulatory network occurs at the subcellular scale, wherein auxin mediates signal transduction into transcriptional programs by triggering the degradation of Aux/IAA transcriptional repressor proteins in the nucleus. However, whether and how auxin movement between the nucleus and the surrounding compartments is regulated remain elusive. Using a fluorescent auxin analog, we show that its diffusion into the nucleus is restricted. By combining mathematical modeling with time course assays on auxin-mediated nuclear signaling and quantitative phenotyping in single plant cell systems, we show that ER-to-nucleus auxin flux represents a major subcellular pathway to directly control nuclear auxin levels. Our findings propose that the homeostatically regulated auxin pool in the ER and ER-to-nucleus auxin fluxes underpin auxin-mediated downstream responses in plant cells. : Middleton et al. study how the plant phytohormone auxin enters the nucleus by using quantitative phenotyping in single plant cell systems and bespoke mathematical models that relate controlled perturbations to experimentally measurable responses. Their findings show that auxin predominantly enters the nucleus via the endoplasmic reticulum. Keywords: auxin, auxin sensor, endoplasmic reticulum, nucleus, auxin flux, fluorescent aux, mathematical modeling, protoplasts, microscopy, single cells |
| Databáze: | OpenAIRE |
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