Chronic endothelial dysfunction after oversized coronary balloon angioplasty in pigs: a 12-week follow-up of coronary vasoreactivity in vivo and in vitro
| Autor: | E. Neil Schachter, Valentin Fuster, John T. Fallon, Francisco Antonio Helfenstein Fonseca, Adrian Padurean, James H. Chesebro, Maria C. O. Izar, Richard L. Gallo, Juan J. Badimon |
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| Rok vydání: | 2001 |
| Předmět: |
Serotonin
medicine.medical_specialty Time Factors Vascular smooth muscle Endothelium Swine Vasodilator Agents medicine.medical_treatment Vasodilation In Vitro Techniques Bradykinin Nitroglycerin In vivo Internal medicine Angioplasty Animals Vasoconstrictor Agents Medicine Nitric Oxide Donors Endothelial dysfunction business.industry Vasospasm Equipment Design medicine.disease Coronary Vessels Vasomotor System medicine.anatomical_structure Anesthesia Cardiology Endothelium Vascular medicine.symptom Cardiology and Cardiovascular Medicine business Angioplasty Balloon Vasoconstriction Follow-Up Studies |
| Zdroj: | Atherosclerosis. 154:61-69 |
| ISSN: | 0021-9150 |
| Popis: | Previous studies have reported the development of vasoconstriction immediately after invasive coronary interventions. Other studies in animals have demonstrated that using oversized balloon angioplasty, vasospasm can be suppressed, even in the presence of endothelial denudation due to important structural alteration in vascular smooth muscle. The regenerated endothelium also appears to be impaired chronically by selective attenuation of in vitro endothelial dependent relaxation related to pertussis toxin-sensitive G proteins. The purpose of this investigation was to verify in vivo and in vitro vasoreactivity to bradykinin (BK) and serotonin (5-hydroxytryptamine; 5-HT) (endothelial dependent agonists) as well as to nitroglycerin (NTG) (exogenous nitric oxide donor) at different times after oversized balloon angioplasty intervention ranging from 1 h to 12 weeks, in normal porcine coronary arteries. BK-induced vasodilatation in vivo was impaired acutely, but it was restored after 4 weeks. Serotonin caused vasoconstriction in vivo that was significantly augmented after 12 weeks. Conversely, endothelium-dependent vasodilatation in vitro to BK and 5-HT remained attenuated during the whole period of follow-up. Finally, relaxation elicited by NTG was reduced in the in vivo experiment until the first week after the procedure. Histological analysis showed severe arterial injury, and complete recovery of endothelial coverage after 4 weeks. In conclusion, this experiment supports evidence for the occurrence of the acute attenuation of vasoresponsiveness and chronic endothelial dysfunction following overstretching coronary balloon angioplasty. Abnormal remodeling associated with the severity of injury may contribute to chronic endothelial dysfunction. Differences found between in vivo and in vitro studies also suggest that multiple endogenous influences present in the former can attenuate the greater endothelial dysfunction demonstrated by endothelial assessment in vitro. |
| Databáze: | OpenAIRE |
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