Separation of precursor T cells and Ig-secreting B cells from the large lymphocytic cells of human tonsil
| Autor: | Claude Griscelli, Fritz Daguillard, Ballet Jj, Anne Durandy, Monique Agrapart |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Rosette Formation
Immunoglobulin gamma-Chains T-Lymphocytes Lymphocyte Palatine Tonsil Immunology Cell Separation Epitopes medicine Animals Humans Cytotoxic T cell Secretion Lymphocytes Antibody-Producing Cells B-Lymphocytes CD40 biology Cell Differentiation Molecular biology In vitro Thymidine incorporation medicine.anatomical_structure Biochemistry Tonsil biology.protein Rabbits Antibody |
| Zdroj: | Cellular Immunology. 33:291-296 |
| ISSN: | 0008-8749 |
| Popis: | Large lymphocytic cells of human tonsils concentrating in the lighter fractions of a BSA gradient contained both precursors of E rosette-forming cells and lymphocytes carrying μ and γ determinants. These two subpopulations of cells could be separated by retaining the Ig-carrying cells on an anti-Fab column. Cells which did not bind to the column matured within 3–5 days into E rosette-forming cells. The Ig-carrying cells were eluted by medium supplemented with immunoglobulins and continued to secrete γ determinants in vitro . Both types of cells were also distinguished by different profiles of spontaneous [ 3 H]thymidine incorporation, the persisting capacity for division of the E-rosette-maturing cells suggesting that they represent an earlier stage of lymphocyte maturation (precursor T cells). |
| Databáze: | OpenAIRE |
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