Storage of lactic streptococci. I. Effect of pH on survival and endogenous metabolism in phosphate buffer
| Autor: | G.R. Cowell, S.J. Weese, John A. Koburger |
|---|---|
| Rok vydání: | 1966 |
| Předmět: |
Streptococcus
Phosphate buffered saline Streptococcus lactis Biology Hydrogen-Ion Concentration medicine.disease_cause Phosphate Endogenous metabolism Phosphates chemistry.chemical_compound Biochemistry chemistry Genetics medicine Animal Science and Zoology Glycolysis Streptococcus cremoris Food Science |
| Zdroj: | Journal of dairy science. 49(4) |
| ISSN: | 0022-0302 |
| Popis: | Washed cells of Streptococcus lactis E and Streptococcus cremoris 18 were stored up to ten days at 4C in 0.05 m phosphate buffers in the range of pH 4.5-11.5. Decreased glycolytic activity of the recovered cells, when assayed in milk, was due to loss of viability. Viability was dependent upon length of storage and pH of the suspending buffer. The optimum pH for storage of these organisms was found to be 8.5. Analyses for amino nitrogen indicate that endogenous metabolism is required for maintenance of maximum viability during storage. When aged cells were recovered from the pH 4.5 buffer and resuspended at pH 8.5 for 24 hr, endogenous metabolism was stimulated and a portion of the glycolytic activity restored. |
| Databáze: | OpenAIRE |
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