In vitro culture of stress erythroid progenitors identifies distinct progenitor populations and analogous human progenitors
| Autor: | Yuanting Chen, Dai Chen Wu, Robert F. Paulson, Jie Xiang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Transgene
Immunology Spleen Antigens CD34 Mice Transgenic Biology In Vitro Techniques Biochemistry Colony-Forming Units Assay Hemoglobins Mice Red Cells Iron and Erythropoiesis Antigens CD Stress Physiological hemic and lymphatic diseases Fetal hemoglobin medicine Animals Humans Erythropoiesis gamma-Globins AC133 Antigen Progenitor cell Hypoxia Erythropoietin Progenitor Bone Marrow Transplantation Glycoproteins Erythroid Precursor Cells Fetus Anemia Cell Biology Hematology Cell biology Mice Inbred C57BL Oxygen medicine.anatomical_structure Bone marrow Peptides |
| Zdroj: | Blood. 125(11) |
| ISSN: | 1528-0020 |
| Popis: | Tissue hypoxia induces a systemic response designed to increase oxygen delivery to tissues. One component of this response is increased erythropoiesis. Steady-state erythropoiesis is primarily homeostatic, producing new erythrocytes to replace old erythrocytes removed from circulation by the spleen. In response to anemia, the situation is different. New erythrocytes must be rapidly made to increase hemoglobin levels. At these times, stress erythropoiesis predominates. Stress erythropoiesis is best characterized in the mouse, where it is extramedullary and utilizes progenitors and signals that are distinct from steady-state erythropoiesis. In this report, we use an in vitro culture system that recapitulates the in vivo development of stress erythroid progenitors. We identify cell-surface markers that delineate a series of stress erythroid progenitors with increasing maturity. In addition, we use this in vitro culture system to expand human stress erythroid progenitor cells that express analogous cell-surface markers. Consistent with previous suggestions that human stress erythropoiesis is similar to fetal erythropoiesis, we demonstrate that human stress erythroid progenitors express fetal hemoglobin upon differentiation. These data demonstrate that similar to murine bone marrow, human bone marrow contains cells that can generate BMP4-dependent stress erythroid burst-forming units when cultured under stress erythropoiesis conditions. |
| Databáze: | OpenAIRE |
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