Coordination of cohabiting phage elements supports bacteria–phage cooperation
| Autor: | Olga Stadnyuk, Lev Rabinovich, Gil Azulay, Ilya Borovok, Tal Argov, Nadejda Sigal, Anna Pasechnek, Shai Ran Sapir, Anat A. Herskovits |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Prophages Science viruses 030106 microbiology Phage biology General Physics and Astronomy Virulence Locus (genetics) Genome Viral Biology medicine.disease_cause Genome Article General Biochemistry Genetics and Molecular Biology Bacterial genetics 03 medical and health sciences Bacteriolysis Bacteriocins Listeria monocytogenes Bacteriocin medicine Bacteriophages Amino Acid Sequence lcsh:Science Lysogeny Prophage Genetics Multidisciplinary Sequence Homology Amino Acid food and beverages General Chemistry Chromosomes Bacterial biology.organism_classification 030104 developmental biology Host-Pathogen Interactions Metalloproteases Virus Activation lcsh:Q Pathogens Genome Bacterial Bacteria |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019) Nature Communications |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-019-13296-x |
| Popis: | Bacterial pathogens often carry multiple prophages and other phage-derived elements within their genome, some of which can produce viral particles in response to stress. Listeria monocytogenes 10403S harbors two phage elements in its chromosome, both of which can trigger bacterial lysis under stress: an active prophage (ϕ10403S) that promotes the virulence of its host and can produce infective virions, and a locus encoding phage tail-like bacteriocins. Here, we show that the two phage elements are co-regulated, with the bacteriocin locus controlling the induction of the prophage and thus its activity as a virulence-associated molecular switch. More specifically, a metalloprotease encoded in the bacteriocin locus is upregulated in response to stress and acts as an anti-repressor for CI-like repressors encoded in each phage element. Our results provide molecular insight into the phenomenon of polylysogeny and its intricate adaptation to complex environments. Bacterial pathogens often carry multiple phage-derived elements within their genome. Here, the authors show that two phage elements are co-regulated in Listeria monocytogenes, the first one controlling the induction of the second one, which in turn regulates virulence of their bacterial host. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|