The Effect of Natural Feline Coronavirus Infection on the Host Immune Response: A Whole-Transcriptome Analysis of the Mesenteric Lymph Nodes in Cats with and without Feline Infectious Peritonitis
| Autor: | Marina L. Meli, Carole Burgener, Anja Kipar, Emi N Barker, Séverine Tasker, Alexandra Malbon, Giancarlo Russo |
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| Přispěvatelé: | University of Zurich, Malbon, Alexandra J |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Feline coronavirus 10184 Institute of Veterinary Pathology lcsh:Medicine Biology medicine.disease_cause Article 2726 Microbiology (medical) Pathogenesis Immune system seq cell mediated immunity generation sequencing 2400 General Immunology and Microbiology humoral immunity medicine 1312 Molecular Biology Immunology and Allergy Mesenteric lymph nodes 11434 Center for Clinical Studies Molecular Biology FIP CATS General Immunology and Microbiology lcsh:R Covid19 2725 Infectious Diseases Feline infectious peritonitis Infectious Diseases medicine.anatomical_structure Immunology Humoral immunity biology.protein 11404 Department of Clinical Diagnostics and Services 2723 Immunology and Allergy 570 Life sciences biology RNA next next-generation sequencing Antibody RNA-seq FCoV |
| Zdroj: | Pathogens, Vol 9, Iss 524, p 524 (2020) Pathogens Volume 9 Issue 7 PATHOGENS Malbon, A, Russo, G, Burgener, C, Barker, E N, Meli, M L, Tasker, S & Kipar, A 2020, ' The effect of natural feline coronavirus infection on the host immune response: a whole-transcriptome analysis on the mesenteric lymph nodes of cats with and without feline infectious peritonitis ', Pathogens . https://doi.org/10.3390/pathogens9070524 Malbon, A, Russo, G, Burgener, C, Barker, E N, Meli, M L, Tasker, S & Kipar, A 2020, ' The effect of natural feline coronavirus infection on the host immune response : a whole-transcriptome analysis on the mesenteric lymph nodes of cats with and without feline infectious peritonitis ', Pathogens, vol. 9, no. 7, 524 . https://doi.org/10.3390/pathogens9070524 |
| ISSN: | 2076-0817 |
| DOI: | 10.3390/pathogens9070524 |
| Popis: | Feline infectious peritonitis (FIP) is a coronavirus-induced disease of cats, in which the immune system is known to play a crucial, but complex, role in the pathogenesis. This role is still incompletely understood, with involvement of both host and viral factors. To evaluate differential gene expression and pathway involvement in feline coronavirus (FCoV) infection and FIP, we applied next-generation RNA-sequencing of the mesenteric lymph nodes from cats with naturally-acquired FIP, as well as those with systemic FCoV infection without FIP, and those with neither. Viral infection was associated with upregulation of viral defenses regardless of the disease state, but to a greater degree in FIP. FIP was associated with higher pro-inflammatory pathway enrichment, whilst non-FIP FCoV-positive cats showed lower enrichment of humoral immunity pathways, below that of uninfected cats in the case of immunoglobulin production pathways. This host response is presumed to be protective. In FIP, downregulation of T cell-related processes was observed, which did not occur in non-FIP FCoV-positive cats. These results emphasize the importance of the host&rsquo s immune balance in determining the outcome of the FCoV infection. |
| Databáze: | OpenAIRE |
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