Identification of circulating biomarkers in sera of Plasmodium knowlesi-infected malaria patients – comparison against Plasmodium vivax infection
| Autor: | C.K. Chan, Subash Cb Gopinath, Jesinda P Kerishnan, Yee L Lau, Yin-Ling Wong, Yeng Chen |
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| Jazyk: | angličtina |
| Předmět: |
Adult
Male Antigenicity Plasmodium vivax Antigens Protozoan Sensitivity and Specificity Immunoproteomics Blood serum parasitic diseases medicine Humans Electrophoresis Gel Two-Dimensional Plasmodium knowlesi biology Haptoglobin Malaysia Hemopexin Middle Aged biology.organism_classification medicine.disease Virology Malaria Infectious Diseases Immunology biology.protein Female Biomarkers Research Article |
| Zdroj: | BMC Infectious Diseases |
| ISSN: | 1471-2334 |
| DOI: | 10.1186/s12879-015-0786-2 |
| Popis: | Background Plasmodium knowlesi was identified as the fifth major malaria parasite in humans. It presents severe clinical symptoms and leads to mortality as a result of hyperparasitemia in a short period of time. This study aimed to improve the current understanding of P. knowlesi and identify potential biomarkers for knowlesi malaria. Methods In the present study, we have employed two-dimensional gel electrophoresis-coupled immunoblotting techniques and mass spectrometry to identify novel circulating markers in sera from P. knowlesi-infected patients. Specifically, we have compared serum protein profiles from P. knowlesi-infected patients against those of healthy or P. vivax-infected individuals. Results We identified several immunoreactive proteins in malarial-infected subjects, including alpha-2-HS glycoprotein (AHSG), serotransferrin (TF), complement C3c (C3), hemopexin (HPX), zinc-2-alpha glycoprotein (ZAG1), apolipoprotein A1 (Apo-A1), haptoglobin (HAP), and alpha-1-B-glycoprotein (A1BG). However, only TF and HPX displayed enhanced antigenicity and specificity, suggesting that they might represent valid markers for detecting P. knowlesi infection. Additionally, six P. knowlesi-specific antigens were identified (K15, K16, K28, K29, K30, and K38). Moreover, although HAP antigenicity was observed during P. vivax infection, it was undetectable in P. knowlesi-infected subjects. Conclusions We have demonstrated the application of immunoproteomics approach to identify potential candidate biomarkers for knowlesi malaria infection. |
| Databáze: | OpenAIRE |
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