Glutamate receptor-channel gating. Maximum likelihood analysis of gigaohm seal recordings from locust muscle
| Autor: | Peter N.R. Usherwood, Frank Ball, Mark S.P. Sansom, R. L. Ramsey, S. E. Bates |
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| Rok vydání: | 1990 |
| Předmět: |
Biophysics
Glutamic Acid Grasshoppers Gating In Vitro Techniques Models Biological Ion Channels Glutamates Animals Ion channel Mathematics biology Muscles Autocorrelation Anatomy biology.organism_classification Receptors Neurotransmitter Electrophysiology Kinetics Dwell time Receptors Glutamate Membrane channel Biological system Locust Research Article Communication channel |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/s0006-3495(90)82367-5 |
| Popis: | Gigaohm recordings have been made from glutamate receptor channels in excised, outside-out patches of collagenase-treated locust muscle membrane. The channels in the excised patches exhibit the kinetic state switching first seen in megaohm recordings from intact muscle fibers. Analysis of channel dwell time distributions reveals that the gating mechanism contains at least four open states and at least four closed states. Dwell time autocorrelation function analysis shows that there are at least three gateways linking the open states of the channel with the closed states. A maximum likelihood procedure has been used to fit six different gating models to the single channel data. Of these models, a cooperative model yields the best fit, and accurately predicts most features of the observed channel gating kinetics. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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