Effect of sire and extender on sperm motility and share of live or dead sperm in bulls’ fresh ejaculate and in AI doses after thawing
Autor: | F. Louda, Jan Beran, Luděk Stádník, Jaromír Ducháček, Jaroslav Čítek, Jiří Bezdíček |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
Cultural Studies
endocrine system food.ingredient urogenital system Artificial insemination medicine.medical_treatment Extender Sire Religious studies Biology Sperm Motile spermatozoa law.invention Andrology food law Yolk embryonic structures medicine Sperm motility reproductive and urinary physiology |
Zdroj: | Scopus-Elsevier ResearcherID |
ISSN: | 2363-9822 |
Popis: | The objectives were to evaluate the effects of sire and content of fresh or ionised egg yolk in extenders on sperm motility and share of live and dead sperm in collected ejaculate, in thawed artificial insemination (AI) doses, and during thermodynamic testing compared to extenders not containing egg yolk. Ejaculates were collected once a week from 4 Holstein bulls. Each of the 20 ejaculate samples from each bull was diluted with 4 different extenders. AndroMed and Bioxcell (no egg yolk) and Triladyl and Optidyl (fresh, ionised egg yolk) were used. A total of 640 AI doses were analysed. The volume of samples, sperm concentration, and percentage of motile spermatozoa were evaluated after collection, as was sperm motility after thawing of AI doses and during thermodynamic testing. Percentages of live and dead sperm were also evaluated. The data set was analysed using SAS/STAT 9.1 (SAS Institute Inc., Cary, NC, USA). The results confirmed significant (P |
Databáze: | OpenAIRE |
Externí odkaz: |