Algal–bacterial synergy in treatment of winery wastewater
| Autor: | Oliver Fiehn, Patrick S. Fitzgerald, Jean S. VanderGheynst, Ingrid Gennity, Brendan T. Higgins, Shannon J. Ceballos |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Chlorella sorokiniana lcsh:TD201-500 010504 meteorology & atmospheric sciences biology Management Monitoring Policy and Law Auxenochlorella Bacterial growth biology.organism_classification 01 natural sciences Pollution 03 medical and health sciences 030104 developmental biology lcsh:Water supply for domestic and industrial purposes Wastewater Algae Sewage treatment Green algae Food science Waste Management and Disposal Bacteria 0105 earth and related environmental sciences Water Science and Technology |
| Zdroj: | npj Clean Water, Vol 1, Iss 1, Pp 1-10 (2018) |
| ISSN: | 2059-7037 |
| DOI: | 10.1038/s41545-018-0005-y |
| Popis: | There is significant potential for employing algae in tertiary wastewater treatment, however, little is known about the contribution of algae-bacteria synergy toward treatment performance. This study demonstrates potential synergy in the treatment of three winery wastewater samples. Two strains of green algae, Auxenochlorella protothecoides and Chlorella sorokiniana were tested and each removed > 90% of nitrogen, > 50% of phosphate, and 100% of acetic acid in the wastewater. Both algae strains grew significantly faster on wastewaters compared to growth on minimal media. Organic carbon in the wastewater apparently played a limited role in algal growth enhancement. When cultured on sterile-filtered wastewater, A. protothecoides increased soluble COD loadings in two of the three wastewaters and C. sorokiniana secreted an insoluble film. Culturing algae with the native wastewater microbial community negated the secretion of algal photosynthate, allowing for simultaneous reductions in COD and nutrient concentrations. Both algae species stimulated bacterial growth in a strain-specific way, suggesting unique responses to algal photosynthate. Cofactor auxotrophy for thiamine, cobalamin, and biotin is widespread among algae and these cofactors are typically obtained from bacteria. Sequencing the wastewater microbial community revealed bacteria capable of synthesizing all three cofactors while liquid chromatography with mass spectrometry (LCMS) and bio-assays revealed the presence of thiamine metabolites in the wastewaters. These cofactors likely increased algal growth rates, particularly for A. protothecoides, which cannot synthesize thiamine de-novo but can salvage it from degradation products. Collectively, these results demonstrate that bacteria and algae provided synergistic growth benefits, potentially contributing to higher levels of wastewater treatment than either organism type alone. |
| Databáze: | OpenAIRE |
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