Extreme copy number variation at a tRNA ligase gene affecting phenology and fitness in yellow monkeyflowers
| Autor: | Kayli Anderson, Findley R. Finseth, Patrick J. Monnahan, Mariah McIntosh, Lila Fishman, Evan Macarthur-Waltz, John K. Kelly, Thomas C. Nelson |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mimulus guttatus DNA Copy Number Variations Population Quantitative Trait Loci Mimulus Locus (genetics) Balancing selection 010603 evolutionary biology 01 natural sciences Article 03 medical and health sciences Genetics Copy-number variation Allele education Gene Ecology Evolution Behavior and Systematics Genetic diversity education.field_of_study biology Chromosome Mapping RNA Ligase (ATP) biology.organism_classification 030104 developmental biology Genetics Population Phenotype Genetic Fitness |
| Popis: | Copy number variation (CNV) is a major part of the genetic diversity segregating within populations, but remains poorly understood relative to single nucleotide variation. Here, we report on a tRNA ligase gene (Migut.N02091; RLG1a) exhibiting unprecedented, and fitness-relevant, CNV within an annual population of the yellow monkeyflower Mimulus guttatus. RLG1a variation was associated with multiple traits in pooled population sequencing (PoolSeq) scans of phenotypic and phenological cohorts. Resequencing of inbred lines revealed intermediate-frequency three-copy variants of RLG1a (trip+; 5/35 = 14%), and trip+ lines exhibited elevated RLG1a expression under multiple conditions. trip+ carriers, in addition to being over-represented in late-flowering and large-flowered PoolSeq populations, flowered later under stressful conditions in a greenhouse experiment (p |
| Databáze: | OpenAIRE |
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