Large-Scale Microcarrier Culture of Chinese Perch Brain Cell for Viral Vaccine Production in a Stirred Bioreactor
| Autor: | Hongru Liang, Yinjie Niu, Qiang Lin, Xiaozhe Fu, Ningqiu Li, Luo Xia, Lihui Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Immunology
Spleen Virus Article Microbiology Laboratory flask bioreactor Drug Discovery Siniperca chuatsi Bioreactor medicine Pharmacology (medical) Pharmacology biology Chemistry suspension culture Viral Vaccine Microcarrier biology.organism_classification equipment and supplies microcarrier Infectious Diseases medicine.anatomical_structure Cytodex 1 Medicine CPB cell line Kidney necrosis |
| Zdroj: | Vaccines Volume 9 Issue 9 Vaccines, Vol 9, Iss 1003, p 1003 (2021) |
| ISSN: | 2076-393X |
| DOI: | 10.3390/vaccines9091003 |
| Popis: | Mandarin fish (Siniperca chuatsi) is one of the important cultured fish species in China. Infectious spleen and kidney necrosis virus (ISKNV) and Siniperca Chuatsi rhabdovirus (SCRV) have hindered the development of mandarin fish farming industry. Vaccination is the most effective method for control of viral diseases, however viral vaccine production requires the large-scale culture of cells. Herein, a suspension culture system of Chinese perch brain cell (CPB) was developed on Cytodex 1 microcarrier in a stirred bioreactor. Firstly, CPB cells were cultured using Cytodex 1 microcarrier in 125 mL stirring flasks. With the optimum operational parameters, CPB cells grew well, distributed uniformly, and could fully cover the microcarriers. Then, CPB cells were digested with trypsin and expanded step-by-step with different expansion ratios from the 125 mL stirring bottle to a 500 mL stirring bottle, and finally to a 3-L bioreactor. Results showed that with an expansion ratio of 1:3, we achieved a high cell density level (2.25 × 106 cells/mL) with an efficient use of the microcarriers, which also confirmed the data obtained from the 125 mL stirring flask. Moreover, obvious cytopathic effects (CPE) were observed in the suspended CPB cells post-infection with ISKNV and SCRV. This study provided a large-scale culture system of CPB cells for virus vaccine production. |
| Databáze: | OpenAIRE |
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