The ratio of sTfR/ferritin is associated with the expression level of TfR in rat bone marrow cells after endurance exercise
| Autor: | Qi Gao, Dongsen Liu, Ye Tian, Jiexiu Zhao, Jincheng Xu, Binxiu Zhao |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Male
medicine.medical_specialty Endocrinology Diabetes and Metabolism Iron Clinical Biochemistry Gene Expression Transferrin receptor Bone Marrow Cells Biochemistry Inorganic Chemistry Hemoglobins Total iron-binding capacity Endurance training Internal medicine Physical Conditioning Animal Receptors Transferrin medicine Animals Rats Wistar Soluble transferrin receptor medicine.diagnostic_test biology Transferrin saturation business.industry Reverse Transcriptase Polymerase Chain Reaction Biochemistry (medical) General Medicine Iron deficiency Iron Deficiencies medicine.disease Rats Ferritin Protein Subunits Endocrinology Hematocrit Immunology Ferritins biology.protein Serum iron Exercise Test business |
| Zdroj: | Biological trace element research. 147(1-3) |
| ISSN: | 1559-0720 |
| Popis: | Currently, it is unclear which index of haematological parameters could be used to most easily monitor iron deficiency during endurance training. To address this question, 16 male Wistar rats were randomly assigned to two groups: a sedentary group (n = 8) and an exercised group (n = 8). Initially, animals in the exercise group started running on a treadmill at a rate of 30 m/min, on a 0% grade, for 1 min/session. Running time was gradually increased by 2 min/day. The training plan was one session per day during the initial 2 weeks and two sessions per day during the third to ninth week. At the end of the 9-week experiment, we analysed the blood of the experimental animals for haemoglobin levels, erythrocyte numbers, haematocrit, serum iron levels, total iron binding capacity, transferrin saturation, serum ferritin levels and soluble transferrin receptor (sTfR) levels, and we calculated the ratio of sTfR/ferritin. Erythrocyte numbers, haemoglobin levels and haematocrit values were decreased after 9 weeks of exercise, but sTfR and sTfR/ferritin values were increased (P < 0.01 or P < 0.05). The training regime significantly increased TfR mRNA levels in the bone marrow cells of the exercised rats compared with the sedentary group (1.8 ± 0.5 vs. 1.1 ± 0.2, P < 0.01). These results revealed a significant correlation between TfR levels in the bone marrow cells and the ratio of sTfR/ferritin (r = 0.517; P < 0.01) and sTfR levels (r = 0.206; P < 0.05) in sedentary and exercised rats. In conclusion, we show that sTfR indices and the ratio of sTfR/ferritin could be useful indicators for monitoring iron deficiency during endurance training. |
| Databáze: | OpenAIRE |
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