Interaction of a Lens Cell Transcription Factor with the Proximal Domain of the Mouse γF-Crystallin Promoter
| Autor: | Liu, Q R, Tini, M, Tsui, L C, Breitman, M L |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Gene expression regulation
Dna mutational analysis Base Sequence Lens crystalline - physiology Molecular Sequence Data Oligonucleotides Nuclear Proteins Cell Biology Regulatory Sequences Nucleic Acid Crystallins Rats Mice Sequence Homology Nucleic Acid Biology - biochemistry biology - cytology and histology Lens Crystalline Animals Humans Oligonucleotides - chemistry sense organs Promoter Regions Genetic Molecular Biology Research Article Protein Binding Transcription Factors |
| Zdroj: | Molecular and Cellular Biology. 11:1531-1537 |
| ISSN: | 1098-5549 |
| DOI: | 10.1128/mcb.11.3.1531-1537.1991 |
| Popis: | The elements regulating lens-specific expression of the mouse γF-crystallin gene were examined. Here we show that mouse γF-crystallin sequences -67 to +45 contain a low basal level of lens-specific promoter activity and that sequences -67 to -25, which are highly conserved among different γ-crystallin genes, are able to function as a strong transcriptional activator when duplicated and placed upstream of the TATA box. We also show that nuclear factors from lens and nonlens cells are able to form different complexes with sequences centered at -46 to -36 and demonstrate that binding of the factor from lens cells correlates with lens-specific promoter activity of the mouse γF-crystallin gene. link_to_OA_fulltext |
| Databáze: | OpenAIRE |
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