Electronic Detection of DNA Hybridization by Coupling Organic Field-Effect Transistor-Based Sensors and Hairpin-Shaped Probes
| Autor: | Ambra Giannetti, Corrado Napoli, Annalisa Bonfiglio, Sara Tombelli, Stefano Lai, Francesco Baldini, Massimo Barbaro |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Materials science
02 engineering and technology lcsh:Chemical technology Target concentration 01 natural sciences Biochemistry Article Analytical Chemistry law.invention Transduction (genetics) law Molecular beacon lcsh:TP1-1185 Electrical and Electronic Engineering DNA hybridization detection Instrumentation electronic sensors organic field-effect transistors Organic field-effect transistor business.industry DNA–DNA hybridization 010401 analytical chemistry Transistor 021001 nanoscience & nanotechnology Atomic and Molecular Physics and Optics 0104 chemical sciences hairpin-shaped oligonucleotides Optoelectronics 0210 nano-technology business Selectivity |
| Zdroj: | Sensors (Basel, Switzerland) Sensors; Volume 18; Issue 4; Pages: 990 Sensors (Basel) 18 (2018). doi:10.3390/s18040990 info:cnr-pdr/source/autori:C. Napoli, S. Lai, A. Giannetti, S. Tombelli, F. Baldini, M. Barbaro, A. Bonfiglio/titolo:Electronic Detection of DNA Hybridization by Coupling Organic Field-Effect Transistor-Based Sensors and Hairpin-Shaped Probes/doi:10.3390%2Fs18040990/rivista:Sensors (Basel)/anno:2018/pagina_da:/pagina_a:/intervallo_pagine:/volume:18 Sensors, Vol 18, Iss 4, p 990 (2018) |
| ISSN: | 1424-8220 |
| Popis: | In this paper, the electronic transduction of DNA hybridization is presented by coupling organic charge-modulated field-effect transistors (OCMFETs) and hairpin-shaped probes. These probes have shown interesting properties in terms of sensitivity and selectivity in other kinds of assays, in the form of molecular beacons (MBs). Their integration with organic-transistor based sensors, never explored before, paves the way to a new class of low-cost, easy-to-use, and portable genetic sensors with enhanced performances. Thanks to the peculiar characteristics of the employed sensor, measurements can be performed at relatively high ionic strengths, thus optimizing the probes’ functionality without affecting the detection ability of the device. A complete electrical characterization of the sensor is reported, including calibration with different target concentrations in the measurement environment and selectivity evaluation. In particular, DNA hybridization detection for target concentration as low as 100 pM is demonstrated. |
| Databáze: | OpenAIRE |
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