MHC class II, antigen presentation and tumor necrosis factor in renal tubular epithelial cells
| Autor: | Vicki E. Kelley, Anthony M. Jevnikar, Rudolf P. Wuthrich, Mary A. Yui, Laurie H. Glimcher, Stephanie E. Dumas |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
medicine.medical_specialty
T cell Antigen presentation Gene Expression Biology Epithelium Cell Line MHC class II antigen Immune system Antigen Internal medicine medicine Humans Antigen-presenting cell MHC class II Tumor Necrosis Factor-alpha Histocompatibility Antigens Class II Epithelial Cells Cell Transformation Viral Molecular biology Clone Cells Kidney Tubules medicine.anatomical_structure Endocrinology Nephrology Cell culture biology.protein |
| Zdroj: | Kidney International. 37:783-792 |
| ISSN: | 0085-2538 |
| Popis: | MHC class II, antigen presentation and tumor necrosis factor in renal tubular epithelial cells. Proximal tubular (PT) epithelial cells express MHC class II (la) antigens in immunologically-mediated renal injury. To study the role of PT as accessory cells, we generated several murine PT-like epithelial cell lines by transformation with origin-defective SV40 DNA. These transformed cell lines display typical alkaline phosphatase and γglutamyl-transpeptidase enzyme activity, proliferation to epidermal growth factor (EGF) and sodium-dependent glucose uptake. Clonal lines of transformed tubular cells from both normal C3H/FeJ and autoimmune MRL-lpr mice do not constitutively express la antigens or mRNA for class II. However, stimulation with recombinant interferon-γ (rIFN-γ) induces la mRNA and surface product in the cell lines. These la-positive cells can process and present hen egg-white lysozyme (HEL) to antigen-specific Iak-restricted T cell hybrids. Unstimulated tubular cells do not express detectable IL-1α, IL-1β, TNF-α, or IL-6 mRNA. However, stimulation with IL-1α or LPS induces TNF-α transcripts. We conclude that these cell lines have characteristics most consistent with a proximal tubular origin. They also bear characteristics of accessory cells such as processing and presentation of antigen and TNF-α gene expression. We speculate that PT have the capacity to participate in the pathogenesis of immune renal injury. |
| Databáze: | OpenAIRE |
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