Low-Density Lipoproteins Oxidized After Intestinal Ischemia/Reperfusion in Rats
| Autor: | Mustafa Comert, Serefden Acikgoz, Gamze Yurdakan, Emine Yilmaz Sipahi, Ishak Ozel Tekin |
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| Přispěvatelé: | Zonguldak Bülent Ecevit Üniversitesi |
| Rok vydání: | 2009 |
| Předmět: |
Male
medicine.medical_specialty intestinal ischemia/reperfusion Ischemia Nitric Oxide Synthase Type II medicine.disease_cause Nitric oxide Rats Sprague-Dawley Superoxide dismutase Lipid peroxidation chemistry.chemical_compound Ileum Mesenteric Artery Superior Malondialdehyde Internal medicine Mesenteric Vascular Occlusion medicine Animals Lung biology Superoxide Dismutase lipid peroxidation medicine.disease Rats Lipoproteins LDL Nitric oxide synthase Endocrinology Liver chemistry Biochemistry Reperfusion Injury biology.protein Female Surgery oxidized low-density lipoproteins Reperfusion injury Oxidative stress |
| Zdroj: | Journal of Surgical Research. 157:e47-e54 |
| ISSN: | 0022-4804 |
| Popis: | Background: Intestinal ischemia/reperfusion (I/R) is a complex phenomenon causing destruction of both local and remote tissues, as well as multiple-organ failure. We investigated the role of lipid peroxidation in damage to intestinal, liver, and lung tissues in this pathology. Materials and Methods: The superior mesenteric artery was blocked for 30 min followed by 24 h of reperfusion. Tissues were removed and the presence of oxidized LDL, the activities of the superoxide dismutase enzyme, malondialdehyde levels, and inducible nitric oxide synthase expression were each evaluated in the intestinal, liver, and lung tissues. Results: While there was no staining in the control group tissues, ischemia/reperfusion resulted in positive oxidized LDL staining in all of the I/R test group tissue samples. Inducible nitric oxide synthase expression was significantly increased in the ischemia/reperfusion group tissues. Compared with those of the control group rats, the ischemia/reperfusion group tissues showed significantly higher malondialdehyde levels and lower superoxide dismutase activities. Conclusions: This study demonstrated for the first time that oxidized LDL accumulated in the terminal ileum, liver, and lung tissues after intestinal ischemia/reperfusion. This occurrence (or the presence of oxidized LDL) may be an indicator of ongoing oxidative stress and enhanced lipid peroxidation. Augmentation of inducible nitric oxide synthase expression may play a role in progression of inflammation and LDL oxidation. These data support the hypothesis that cellular oxidative stress is a critical step in reperfusion-mediated injury in both the intestine and end organs, and that antioxidant strategies may provide organ protection in patients with reperfusion injury, at least through affecting interaction with free radicals, nitric oxide, and oxidized LDL. © 2009 Elsevier Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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