Resveratrol supplementation and cryopreservation of buck semen
| Autor: | Salvatore Pau, Laura Falchi, Maria Teresa Zedda, Luisa Bogliolo, I. Pivato |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Male
Motility Semen Resveratrol General Biochemistry Genetics and Molecular Biology Cryopreservation law.invention Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine law Animals Humans Acrosome 030219 obstetrics & reproductive medicine TUNEL assay Goats Extender 0402 animal and dairy science 04 agricultural and veterinary sciences General Medicine Spermatozoa 040201 dairy & animal science chemistry Dietary Supplements Sperm Motility DNA fragmentation General Agricultural and Biological Sciences Semen Preservation |
| Zdroj: | Cryobiology. 95:60-67 |
| ISSN: | 0011-2240 |
| Popis: | Resveratrol (Resv) was tested to assess its effects on buck semen freezability. Ejaculates of 4 bucks were collected, washed and diluted in a commercial extender at 30 °C. Extended semen was divided into 4 aliquots supplemented with increasing concentrations of Resv: 0 μM (control); 10 μM; 25 μM and 50 μM. Aliquots were cooled to 4 °C in 5h and frozen in LN2. Thawing was performed at 37 °C for 30 s. At the 3 stages of the experiment (30 °C, 4 °C, thawing), motility (CASA), osmotic resistance (Hos test) and integrity of cytoplasm and acrosome membranes (PI/PSA staining) were assessed. Moreover, in thawed samples, the oxidative status (MDA assay) and early apoptosis (DNA fragmentation by TUNEL assay) were evaluated. Resveratrol supplementation did not affect most of the motility parameters analysed, except for total motility, ALH (lateral head displacement) and velocity distribution (P 0.05). In thawed spermatozoa, the oxidative status was not preserved by Resv (P > 0.05) while early apoptosis, was significantly decreased in the 50 μM Resv group (P |
| Databáze: | OpenAIRE |
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