Dynamic Imaging of T Cell-Parasite Interactions in the Brains of Mice Chronically Infected withToxoplasma gondii
| Autor: | Tatyana Chtanova, Giel G. van Dooren, Marie Schaeffer, Paul Herzmark, Badrinath Roysam, Boris Striepen, Ying Chen, Seong-Ji Han, Ellen A. Robey |
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| Rok vydání: | 2009 |
| Předmět: |
CD4-Positive T-Lymphocytes
T cell Immunology Antigen-Presenting Cells CD8-Positive T-Lymphocytes Host-Parasite Interactions Mice Immune system Antigen Image Processing Computer-Assisted medicine Animals Immunology and Allergy Cytotoxic T cell Antigen-presenting cell Microscopy Confocal biology Intracellular parasite Brain Toxoplasma gondii Flow Cytometry biology.organism_classification Virology Toxoplasmosis Animal medicine.anatomical_structure Microscopy Fluorescence Toxoplasmosis Cerebral Chronic Disease Astrocyte |
| Zdroj: | The Journal of Immunology. 182:6379-6393 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | The intracellular parasite Toxoplasma gondii can establish persistent infection in the brain of a mammalian host, a standoff that involves the active participation of host CD8 T cells to control infection. CD8 T cells generally protect against intracellular pathogens by local delivery of effector molecules upon recognition of specific pathogen Ags on invaded host cells. However, the interactions between CD8 T cells, T. gondii, and APCs in the brain have not yet been examined. In this study we have used a mouse infection model in conjunction with two-photon microscopy of living brain tissue and confocal microscopy of fixed brain sections to examine the interactions between CD8 T cells, parasites, and APCs from chronically infected mice. We found that Ag-specific CD8 T cells were recruited to the brains of infected mice and persisted there in the presence of ongoing Ag recognition. Cerebral CD8 T cells made transient contacts with granuloma-like structures containing parasites and with individual CD11b+ APCs, including some that did not contain parasites. In contrast, T cells ignored intact Ag-bearing cysts and did not contact astrocytes or neurons, including neurons containing parasites or cysts. Our data represent the first direct observation of the dynamics of T cell-parasite interactions within living tissue and provide a new perspective for understanding immune responses to persistent pathogens in the brain. |
| Databáze: | OpenAIRE |
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