Possible contribution of follicular interleukin-1beta to nitric oxide generation in human pre-ovulatory follicles
| Autor: | Toshinobu Tanaka, Hideya Kodama, Meng Tao, Hideto Hirano, Satoko Kagabu, Masanori Murata, Yasushi Shimizu, Jun Fukuda |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Ovulation endocrine system medicine.medical_specialty Cell Count Ovary Fertilization in Vitro Biology Nitric Oxide Chorionic Gonadotropin Follicular cell Nitric oxide chemistry.chemical_compound Ovarian Follicle 2-Naphthylamine Internal medicine Follicular phase medicine Humans Nitrite Ovarian follicle Nitrites Progesterone Fluorescent Dyes Nitrates Estradiol Rehabilitation Obstetrics and Gynecology Hair follicle Follicular fluid Follicular Fluid Spectrometry Fluorescence medicine.anatomical_structure Endocrinology Reproductive Medicine chemistry Oocytes Female Interleukin-1 |
| Zdroj: | Human Reproduction. 12:2220-2225 |
| ISSN: | 1460-2350 0268-1161 |
| Popis: | The aim of this study was to investigate the relationships between follicular nitric oxide (NO) metabolite concentrations and several related variables, with special reference to follicular interleukin-1beta (IL-1beta). The follicular fluid from the leading and secondary follicles was collected individually from 20 women undergoing in-vitro fertilization (IVF) treatment, and the concentrations of nitrite (NO2-) and nitrate (NO3-) were determined fluorometrically using 2,3-diaminonaphthalene. Both follicular nitrite (r = 0.42, P < 0.01) and nitrate (r = 0.49, P < 0.001) were found to be significantly correlated with follicular IL-1beta concentrations. There were also significant positive correlations between follicular nitrate and the number of oocytes retrieved (P < 0.01) and serum oestradiol concentration on the day of human chorionic gonadotrophin (HCG) administration (P < 0.05). When follicular cells were incubated in vitro with 10 ng/ml of IL-1beta for 24 h, nitrate generation was significantly (P < 0.01) elevated compared with the control. In conclusion, our study demonstrates that follicular IL-1beta and the number of developing follicles are significant variables that affect follicular NO concentrations, and points to the possible contribution of IL-1beta to NO generation in human preovulatory follicles. |
| Databáze: | OpenAIRE |
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