Knockdown of P3H4 inhibits proliferation and invasion of bladder cancer
| Autor: | Zhiguo Zhang, Hui Pang, Lin Hao, Jun-Jie Zhang, Kun Pang, Rongsheng Zhou, Zhenduo Shi, Houguang He, Conghui Han |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Aging Biology Autoantigens Cell Movement Transcription (biology) Cell Line Tumor Humans Gene silencing Neoplasm Invasiveness Transcription factor transcription factor Aged Cell Proliferation Carcinoma Transitional Cell Gene knockdown Proto-Oncogene Proteins c-ets Oncogene ETV4 Cell growth Cell Cycle Promoter Cell Biology Middle Aged Cell cycle P3H4 Urinary Bladder Neoplasms Gene Knockdown Techniques Cancer research bladder cancer Female Research Paper |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| DOI: | 10.18632/aging.102732 |
| Popis: | The prolyl 3-hydroxylase family member 4 (P3H4) (alias SC65) is implicated in a variety of physiological and pathological processes. However, little is known about the role of P3H4 in tumors. This study aimed to investigate the role of P3H4 in bladder cancer (BC) and the regulatory mechanisms that influence its expression. P3H4 was highly expressed in BC tissues. Knockdown of P3H4 inhibited BC cell proliferation, cell cycle, migration and invasion in vitro, and inhibited BC growth in vivo. We also found that ETV4 bound directly to the promoter region of P3H4 and activated its transcription. Furthermore, overexpression of ETV4 rescued the inhibition of proliferation and invasion induced by PH4 silencing. ETV4 was significantly overexpressed in the BC tissues. In conclusion, P3H4 functioned an oncogene role in BC progression, and ETV4 bound directly to the P3H4 promoter region to regulate its transcription. |
| Databáze: | OpenAIRE |
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