Detection of in vivo genotoxicity of 3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H]-furanone (MX) by the alkaline single cell gel electrophoresis (Comet) assay in multiple mouse organs
| Autor: | Naohide Kinae, Naonori Matsusaka, Fusako Izumiyama, Mie Watanabe-Akanuma, Shuji Tsuda, Emi Nishidate, YūF. Sasaki |
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| Rok vydání: | 1997 |
| Předmět: |
Electrophoresis
Male DNA damage Health Toxicology and Mutagenesis Urinary Bladder Ileum Biology Kidney medicine.disease_cause Mice Bone Marrow In vivo Genetics medicine Animals Furans Lung Gel electrophoresis Mutagenicity Tests Brain Hydrogen-Ion Concentration Molecular biology Comet assay medicine.anatomical_structure Liver Organ Specificity Digestive System Spleen Genotoxicity DNA Damage Mutagens Homogenization (biology) |
| Zdroj: | Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 393:47-53 |
| ISSN: | 1383-5718 |
| DOI: | 10.1016/s1383-5718(97)00085-5 |
| Popis: | We tested the genotoxicity of 3-chloro-4-(dichloromethyl)-5-hydroxy-2[5 H ]-furanone (MX) in the mouse in 6 organs (liver, lung, kidney, brain, spleen, and bone marrow) and in the mucosa of stomach, jejunum, ileum, colon, and bladder using the alkaline single-cell gel electrophoresis (SCG) (Comet) assay modified by us. Mice were sacrificed 1, 3, 6, and 24 h after oral administration of the mutagen at 100 mg/kg. MX yielded statistically significant DNA damage in the liver, kidney, lung, and brain and in all the mucosa samples. While DNA damage persisted in the gastrointestinal and urinary tract for 6–24 h after a single oral dosing, it peaked in the liver at 1 h and returned to almost the control level at 3 h. Our present results suggest that MX is genotoxic for various mouse organs, but not for the hematopoietic system, and that the alkaline SCG assay with a homogenization technique can be used to predict genotoxicity in the gastrointestinal and urinary tracts. |
| Databáze: | OpenAIRE |
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