Characterization, cloning and sequence analysis of the inducible Ochrobactrum anthropi AmpC beta-lactamase
| Autor: | Peter M. Bennett, Matthew B. Avison, Catherine S. Higgins, Lee Jamieson, Alan M. Simm, Timothy R. Walsh |
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| Rok vydání: | 2001 |
| Předmět: |
Microbiology (medical)
Ochrobactrum anthropi Sequence analysis Penicillin Resistance Molecular Sequence Data Microbial Sensitivity Tests Molecular cloning beta-Lactams medicine.disease_cause beta-Lactamases Microbiology Bacterial Proteins polycyclic compounds medicine Pharmacology (medical) Amino Acid Sequence Cloning Molecular Escherichia coli Phylogeny Antibacterial agent Pharmacology Cloning Sinorhizobium meliloti Base Sequence Sequence Homology Amino Acid biology biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification Enterobacteriaceae Drug Resistance Multiple Anti-Bacterial Agents Infectious Diseases Enzyme Induction bacteria |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 47:745-754 |
| ISSN: | 1460-2091 |
| Popis: | Ochrobactrum anthropi is resistant to most cephalosporins and penicillins due, at least in part, to the inducible expression of a single beta-lactamase. The beta-lactamase gene has been cloned and sequenced. It encodes an AmpC-type class 1 serine active-site enzyme that hydrolyses mainly cephalosporins and is resistant to inhibition by clavulanic acid. Expression of the ampC gene is inducible via a typical AmpR regulator, which is encoded upstream of ampC. Inducible expression is retained following cloning of O. anthropi ampR-ampC into Escherichia coli, confirming that the signal for AmpR activation in O. anthropi is the same as that used in the Enterobacteriaceae. This is the first reported example of an AmpC beta-lactamase outside of the gamma-subdivision of the bacterial kingdom. Genomic searches of other non-gamma-subdivision bacteria revealed a homologous ampR-ampC cluster in the plant symbiont, Sinorhizobium meliloti. |
| Databáze: | OpenAIRE |
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