Complex adenovirus-vectored vaccine protects guinea pigs from three strains of Marburg virus challenges
Autor: | Min Luo, Stephen B. Deitz, Michael Hevey, Laure Y. Juompan, Charles M. Trubey, Kevin M. Moore, Jan Woraratanadharm, Nicholas U. Raja, Hong Yu, Danher Wang, John Y. Dong, Benjamin M. Swain |
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Rok vydání: | 2006 |
Předmět: |
Injections
Subcutaneous Genetic Vectors Guinea Pigs Molecular Sequence Data Dose-Response Relationship Immunologic Biology medicine.disease_cause Antibodies Viral law.invention Adenoviridae Cell Line Marburg virus Mice Viral Envelope Proteins law Virology Chlorocebus aethiops medicine Animals Humans Marburg Virus Disease Challenge Amino Acid Sequence Ebola Vaccines Antigens Viral Vaccines Synthetic Ci67 Ebola virus Musoke Vaccination Ravn Genetic Therapy Recombinant Proteins Marburgvirus Recombinant DNA biology.protein cAdVax Glycoprotein Antibody Vaccine Sequence Alignment Viral Fusion Proteins Injections Intraperitoneal |
Zdroj: | Virology. 353(2):324-332 |
ISSN: | 0042-6822 |
DOI: | 10.1016/j.virol.2006.05.033 |
Popis: | The Marburg virus (MARV), an African filovirus closely related to the Ebola virus, causes a deadly hemorrhagic fever in humans, with up to 90% mortality. Currently, treatment of disease is only supportive, and no vaccines are available to prevent spread of MARV infections. In order to address this need, we have developed and characterized a novel recombinant vaccine that utilizes a single complex adenovirus-vectored vaccine (cAdVax) to overexpress a MARV glycoprotein (GP) fusion protein derived from the Musoke and Ci67 strains of MARV. Vaccination with the cAdVaxM(fus) vaccine led to efficient production of MARV-specific antibodies in both mice and guinea pigs. Significantly, guinea pigs vaccinated with at least 5 × 107 pfu of cAdVaxM(fus) vaccine were 100% protected against lethal challenges by the Musoke, Ci67 and Ravn strains of MARV, making it a vaccine with trivalent protective efficacy. Therefore, the cAdVaxM(fus) vaccine serves as a promising vaccine candidate to prevent and contain multi-strain infections by MARV. |
Databáze: | OpenAIRE |
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