Complex adenovirus-vectored vaccine protects guinea pigs from three strains of Marburg virus challenges

Autor: Min Luo, Stephen B. Deitz, Michael Hevey, Laure Y. Juompan, Charles M. Trubey, Kevin M. Moore, Jan Woraratanadharm, Nicholas U. Raja, Hong Yu, Danher Wang, John Y. Dong, Benjamin M. Swain
Rok vydání: 2006
Předmět:
Zdroj: Virology. 353(2):324-332
ISSN: 0042-6822
DOI: 10.1016/j.virol.2006.05.033
Popis: The Marburg virus (MARV), an African filovirus closely related to the Ebola virus, causes a deadly hemorrhagic fever in humans, with up to 90% mortality. Currently, treatment of disease is only supportive, and no vaccines are available to prevent spread of MARV infections. In order to address this need, we have developed and characterized a novel recombinant vaccine that utilizes a single complex adenovirus-vectored vaccine (cAdVax) to overexpress a MARV glycoprotein (GP) fusion protein derived from the Musoke and Ci67 strains of MARV. Vaccination with the cAdVaxM(fus) vaccine led to efficient production of MARV-specific antibodies in both mice and guinea pigs. Significantly, guinea pigs vaccinated with at least 5 × 107 pfu of cAdVaxM(fus) vaccine were 100% protected against lethal challenges by the Musoke, Ci67 and Ravn strains of MARV, making it a vaccine with trivalent protective efficacy. Therefore, the cAdVaxM(fus) vaccine serves as a promising vaccine candidate to prevent and contain multi-strain infections by MARV.
Databáze: OpenAIRE