Osmoregulation in larvae and juveniles of two recently separated Macrobrachium species: Expression patterns of ion transporter genes
| Autor: | Nesrine Boudour-Boucheker, Viviane Boulo, Guy Charmantier, Klaus Anger, Mireille Charmantier-Daures, Catherine Lorin-Nebel |
|---|---|
| Přispěvatelé: | MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), Lagons, Ecosystèmes et Aquaculture Durable [Nouméa] (LEAD), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0106 biological sciences
0301 basic medicine Salinity Branchial chamber Physiology Ontogeny [SDE.MCG]Environmental Sciences/Global Changes Zoology V-type H+-ATPase 01 natural sciences Biochemistry Acclimatization 03 medical and health sciences Osmoregulation [SDV.EE.ECO]Life Sciences [q-bio]/Ecology environment/Ecosystems parasitic diseases Animals 14. Life underwater Molecular Biology Larva Ion Transport Macrobrachium biology Ecology Na+/K+-ATPase 010604 marine biology & hydrobiology biology.organism_classification Crustacean 6. Clean water Shrimp Crustaceans 030104 developmental biology NHE3 transporter Palaemonidae Sodium-Potassium-Exchanging ATPase [SDE.BE]Environmental Sciences/Biodiversity and Ecology |
| Zdroj: | Comparative Biochemistry and Physiology-Part A: Molecular and Integrative Physiology Comparative Biochemistry and Physiology-Part A: Molecular and Integrative Physiology, Elsevier, 2016, 195, pp.39-45. ⟨10.1016/j.cbpa.2016.02.005⟩ Comparative Biochemistry And Physiology A-molecular & Integrative Physiology (1095-6433) (Elsevier Science Inc), 2016-05, Vol. 195, P. 39-45 |
| ISSN: | 1095-6433 |
| DOI: | 10.1016/j.cbpa.2016.02.005⟩ |
| Popis: | International audience; In this comparative study, osmoregulatory mechanisms were analyzed in two closely related species of palaemonid shrimp from Brazil, Macrobrachium pantanalense and Macrobrachium amazonicum. A previous investigation showed that all postembryonic stages of M. pantanalense from inland waters of the Pantanal are able to hyper-osmoregulate in fresh water, while this species was not able to hypo-osmoregulate at high salinities. In M. amazonicum originating from the Amazon estuary, in contrast, all stages are able to hypo-osmoregulate, but only first-stage larvae, late juveniles and adults are able to hyper-osmoregulate in fresh water. The underlying molecular mechanisms of these physiological differences have not been known. We therefore investigated the expression patterns of three ion transporters (NKA α-subunit, VHA B-subunit and NHE3) following differential salinity acclimation in different ontogenetic stages (stage-V larvae, juveniles) of both species. Larval NKAα expression was at both salinities significantly higher in M. pantanalense than in M. amazonicum, whereas no difference was noted in juveniles. VHA was also more expressed in larvae of M. pantanalense than in those of M. amazonicum. When NHE3 expression is compared between the larvae of the two species, further salinity-related differences were observed, with generally higher expression in the inland species. Overall, a high expression of ion pumps in M. pantanalense suggests an evolutionary key role of these transporters in freshwater invasion. |
| Databáze: | OpenAIRE |
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