Field evaluation of the Abbott ARCHITECT HIV Ag/Ab Combo immunoassay
| Autor: | Anfumbom Kfutwah, Hélène Valérie Ngono, Véronique Lemée, Fabienne De Oliveira, Jean-Christophe Plantier, Richard Njouom |
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| Rok vydání: | 2013 |
| Předmět: |
Adult
Male Adolescent HIV Antigens HIV diagnosis Population Human immunodeficiency virus (HIV) HIV Infections Biology HIV Antibodies medicine.disease_cause Sensitivity and Specificity Young Adult Virology medicine Fourth generation Humans Cameroon education Child Aged Aged 80 and over Immunoassay education.field_of_study medicine.diagnostic_test Diagnostic Tests Routine virus diseases Central africa Diagnostic test Infant Middle Aged Infectious Diseases Child Preschool HIV-2 HIV-1 Female France |
| Zdroj: | Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology. 58 |
| ISSN: | 1873-5967 |
| Popis: | Background Fourth generation assays for HIV diagnosis are progressively being introduced into routine services, due to their improvement of diagnosis. In spite of this, HIV diagnosis remains a challenge in sub-Saharan Africa, due to false positive reactivity. There is a continuous need for field evaluations and routine validations of fourth generation HIV tests in African populations. Objectives Evaluate the performances of the ARCHITECT HIV Ag/Ab kit (Abbott) in a population living in an African setting-Cameroon compared to a population living in a European setting-France. Study design 645 HIV samples from both France and Cameroon were evaluated. The positive panel (378 samples) included a diverse series of HIV-1 variants (groups M, N, O, and P) as well as HIV-2 samples. Results were compared to original diagnosis done with other 4th generation assays (AxSYM HIV Ag/Ab (Abbott) and Vidas HIV DUO QUICK) (bioMerieux). Results Sensitivity of the ARCHITECT was 100% in both sites. It diagnosed all variants of the panel with different reactivity profiles following strain diversity. A wider range of reactivity was observed for group O. Specificity was slightly lower (97.6%) in Cameroon than in France (98.6%), probably due to a higher rate of false positive reactivity. ARCHITECT HIV Ag/Ab assay had high performances in clinical sensitivity and specificity and is adapted to the wide genetic diversity of viruses circulating in West Central Africa. Conclusion Our results further highlight the need to evaluate HIV diagnostic tests before introduction into routine diagnostic services both in the North and in the South. |
| Databáze: | OpenAIRE |
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