Cutting Edge: Identification of GL50, a Novel B7-Like Protein That Functionally Binds to ICOS Receptor
| Autor: | Heather Finnerty, Mary Collins, Sharon E. Hunter, Vicki Spaulding, Vincent Ling, Joy S. Miyashiro, Lynette A. Fouser, John P. Leonard, Richard Zollner, Jenifer L. Thomas, Kenneth Jacobs, Kevin M. Bean, Paul W. Wu |
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| Rok vydání: | 2000 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte DNA Complementary Transcription Genetic CD3 Molecular Sequence Data Immunology Ligands Inducible T-Cell Co-Stimulator Protein Inducible T-Cell Co-Stimulator Ligand Mice Antigens CD Complementary DNA Lymphocyte costimulation Tumor Cells Cultured Animals Humans Immunology and Allergy Amino Acid Sequence Cloning Molecular Receptor Mice Inbred BALB C Membrane Glycoproteins biology Proteins Blotting Northern Ligand (biochemistry) Molecular biology Mice Inbred C57BL ICOS LIGAND B7-1 Antigen biology.protein B7-2 Antigen Lymph Nodes Sequence Alignment |
| Zdroj: | The Journal of Immunology. 164:1653-1657 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | By the genetic selection of mouse cDNAs encoding secreted proteins, a B7-like cDNA clone termed mouse GL50 (mGL50) was isolated encoding a 322-aa polypeptide identical with B7h. Isolation of the human ortholog of this cDNA (hGL50) revealed a coding sequence of 309 aa residues with 42% sequence identity with mGL50. Northern analysis indicated GL50 to be present in many tissues including lymphoid, embryonic yolk sac, and fetal liver samples. Of the CD28, CTLA4, and ICOS fusion constructs tested, flow cytometric analysis demonstrated only mouse ICOS-IgG binding to mGL50 cell transfectants. Subsequent phenotyping demonstrated high levels of ICOS ligand staining on splenic CD19+ B cells and low levels on CD3+ T cells. These results indicate that GL50 is a specific ligand for the ICOS receptor and suggest that the GL50-ICOS interaction functions in lymphocyte costimulation. |
| Databáze: | OpenAIRE |
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