Enhancing Photosynthetic Production of Glycogen-Rich Biomass for Use as a Fermentation Feedstock
| Autor: | Austin D. Comer, Joshua P. Abraham, Alexander J. Steiner, Travis C. Korosh, Andrew L. Markley, Brian F. Pfleger |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Economics and Econometrics
Commodity chemicals 020209 energy Energy Engineering and Power Technology Biomass lcsh:A 02 engineering and technology Raw material cyanobacteria Article chemistry.chemical_compound 0202 electrical engineering electronic engineering information engineering Food science Glycogen synthase feedstock Glycogen biology Phototroph Renewable Energy Sustainability and the Environment food and beverages 021001 nanoscience & nanotechnology biofuels Fuel Technology chemistry Biofuel glycogen biology.protein Fermentation metabolic engineenng lcsh:General Works 0210 nano-technology |
| Zdroj: | Frontiers in Energy Research, Vol 8 (2020) Front Energy Res |
| DOI: | 10.3389/fenrg.2020.00093/full |
| Popis: | Current sources of fermentation feedstocks, i.e. corn, sugar cane, or plant biomass, fall short of demand for liquid transportation fuels and commodity chemicals in the United States. Aquatic phototrophs including cyanobacteria have the potential to supplement the supply of current fermentable feedstocks. In this strategy, cells are engineered to accumulate storage molecules including glycogen, cellulose, and/or lipid oils that can be extracted from harvested biomass and fed to heterotrophic organisms engineered to produce desired chemical products. In this manuscript, we examine the production of glycogen in the model cyanobacteria, Synechococcus sp. strain PCC 7002, and subsequent conversion of cyanobacterial biomass by an engineered Escherichia coli to octanoic acid as a model product. In effort to maximize glycogen production, we explored the deletion of catabolic enzymes and overexpression of GlgC, an enzyme that catalyzes the first committed step towards glycogen synthesis. We found that deletion of glgP increased final glycogen titers when cells were grown in diurnal light. Overexpression of GlgC led to a temporal increase in glycogen content but not in an overall increase in final titer or content. The best strains were grown, harvested, and used to formulate media for growth of E. coli. The cyanobacterial media was able to support the growth of an engineered E. coli and produce octanoic acid at the same titer as common laboratory media. |
| Databáze: | OpenAIRE |
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