Detection of SARS-CoV-2 from Saliva as Compared to Nasopharyngeal Swabs in Outpatients

Autor:	Christie Vermeiren, Elena Sheldrake, Kyle Vojdani, Lee W. Goneau, Laura Finlay, Kevin Katz, Robert A. Kozak, Jeff Powis, Melissa Desaulnier, Christopher Kandel, Hilary Racher, Janine McCready, Allison McGeer, Jennifer Zheng, Mihaela Anca Serbanescu
Rok vydání:	2020
Předmět:	Adult Male 0301 basic medicine medicine.medical_specialty Saliva Coronavirus disease 2019 (COVID-19) Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) education 030106 microbiology lcsh:QR1-502 Sensitivity and Specificity Article lcsh:Microbiology Specimen Handling 03 medical and health sciences 0302 clinical medicine Paired samples Limit of Detection Nasopharynx Virology Chart review Internal medicine Outpatients medicine Humans Sampling (medicine) Prospective Studies 030212 general & internal medicine Two sample Ontario saliva SARS-CoV-2 business.industry technology industry and agriculture COVID-19 Middle Aged respiratory system nasopharyngeal swab Infectious Diseases COVID-19 Nucleic Acid Testing RNA Viral Female Sample collection business
Zdroj:	Viruses Volume 12 Issue 11 Viruses, Vol 12, Iss 1314, p 1314 (2020)
ISSN:	1999-4915
DOI:	10.3390/v12111314
Popis:	Widely available and easily accessible testing for COVID-19 is a cornerstone of pandemic containment strategies. Nasopharyngeal swabs (NPS) are the currently accepted standard for sample collection but are limited by their need for collection devices and sampling by trained healthcare professionals. The aim of this study was to compare the performance of saliva to NPS in an outpatient setting. This was a prospective study conducted at three centers, which compared the performance of saliva and NPS samples collected at the time of assessment center visit. Samples were tested by real-time reverse transcription polymerase chain reaction and sensitivity and overall agreement determined between saliva and NPS. Clinical data was abstracted by chart review for select study participants. Of the 432 paired samples, 46 were positive for SARS-CoV-2, with seven discordant observed between the two sample types (four individuals testing positive only by NPS and three by saliva only). The observed agreement was 98.4% (kappa coefficient 0.91) and a composite reference standard demonstrated sensitivity of 0.91 and 0.93 for saliva and NPS samples, respectively. On average, the Ct values obtained from saliva as compared to NPS were higher by 2.76. This study demonstrates that saliva performs comparably to NPS for the detection of SARS-CoV-2. Saliva was simple to collect, did not require transport media, and could be tested with equipment readily available at most laboratories. The use of saliva as an acceptable alternative to NPS could support the use of widespread surveillance testing for SARS-CoV-2.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::af4e430b75ec43555866c18c7b4d6c00 https://doi.org/10.3390/v12111314 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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