RYBP and Cbx7 Define Specific Biological Functions of Polycomb Complexes in Mouse Embryonic Stem Cells
| Autor: | Luciano Di Croce, Salvador Aznar Benitah, Luigi Aloia, Lluis Morey, Luca Cozzuto |
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| Rok vydání: | 2013 |
| Předmět: |
Ubiquitin-Protein Ligases
Polycomb-Group Proteins Plasma protein binding macromolecular substances Biology Mutually exclusive events General Biochemistry Genetics and Molecular Biology Proteïnes -- Metabolisme Mice 03 medical and health sciences 0302 clinical medicine Animals Humans PRC1 complex Cél·lules mare embrionàries lcsh:QH301-705.5 Gene Embryonic Stem Cells 030304 developmental biology Polycomb Repressive Complex 1 Genetics Regulation of gene expression 0303 health sciences Genome Gene Expression Regulation Developmental Embryonic stem cell Chromatin Repressor Proteins lcsh:Biology (General) 030220 oncology & carcinogenesis PRC1 Protein Binding |
| Zdroj: | Recercat. Dipósit de la Recerca de Catalunya instname Cell Reports, Vol 3, Iss 1, Pp 60-69 (2013) Cell Reports; Vol 3 |
| ISSN: | 2211-1247 |
| DOI: | 10.1016/j.celrep.2012.11.026 |
| Popis: | The Polycomb repressive complex 1 (PRC1) is required for decisions of stem cell fate. In mouse embryonic stem cells (ESCs), two major variations of PRC1 complex, defined by the mutually exclusive presence of Cbx7 or RYBP, have been identified. Here, we show that although the genomic localization of the Cbx7- and RYBP-containing PRC1 complexes overlaps in certain genes, it can also be mutually exclusive. At the molecular level, Cbx7 is necessary for recruitment of Ring1B to chromatin, whereas RYBP enhances the PRC1 enzymatic activity. Genes occupied by RYBP show lower levels of Ring1B and H2AK119ub and are consequently more highly transcribed than those bound by Cbx7. At the functional level, we show that genes occupied by RYBP are primarily involved in the regulation of metabolism and cell-cycle progression, whereas those bound by Cbx7 predominantly control early-lineage commitment of ESCs. Altogether, our results indicate that different PRC1 subtypes establish a complex pattern of gene regulation that regulates common and nonoverlapping aspects of ESC pluripotency and differentiation. This work was supported by grants from the Spanish "Ministerio de/nEducación y Ciencia" (BFU2010-18692), from AGAUR, and from by European Commission's 7th Framework Program 4DCellFate grant number 277899 to L.D.C.; L.M. was supported by a postdoctoral CRG-Novartis fellowship |
| Databáze: | OpenAIRE |
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