Predictive and Prognostic Significance of Glutathione Levels and DNA Damage in Cervix Cancer Patients Undergoing Radiotherapy
| Autor: | Donald J Fernandes, B.M. Vadhiraja, Mamidipudi Srinivasa Vidyasagar, Maheedhar Kodali, Pu Prakash Saxena, Satish Rao Bola Sadashiva, Dinesh Upadhya, Chilakapati Murali Krishna |
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| Rok vydání: | 2010 |
| Předmět: |
Adult
Cancer Research medicine.medical_specialty Pathology medicine.medical_treatment Brachytherapy Urology Uterine Cervical Neoplasms Antineoplastic Agents Biomarkers Tumor Humans Medicine Radiology Nuclear Medicine and imaging Cervix Aged Cisplatin Cervical cancer Radiation business.industry Dose fractionation Cancer Middle Aged Prognosis medicine.disease Combined Modality Therapy Glutathione Radiation therapy medicine.anatomical_structure Oncology Female Dose Fractionation Radiation business Chemoradiotherapy DNA Damage medicine.drug |
| Zdroj: | International Journal of Radiation Oncology*Biology*Physics. 78:343-349 |
| ISSN: | 0360-3016 |
| Popis: | Purpose To assess the predictive significance of serum glutathione (GSH) and tumor tissue DNA damage in the treatment of cervical cancer patients undergoing chemoradiotherapy. Methods and Materials This study included subjects undergoing hysterectomy (for normal cervix tissue) and cervical cancer patients who underwent conventional concurrent chemoradiotherapy (cisplatin once per week for 5 weeks with concurrent external radiotherapy of 2 Gy per fraction for 5 weeks, followed by two applications of intracavitary brachytherapy once per week after 2 weeks' rest). Blood was collected after two fractions, whereas both blood and tissues were collected after five fractions of radiotherapy in separate groups of subjects. Serum for total GSH content and tissues were processed for single-cell gel electrophoresis (SCGE) assay for DNA damage analysis. Clinical tumor radioresponse was assessed 2 months after the completion of treatment as complete responders (CR) (100% shrinkage), partial responders (PR) (>50%), and nonresponders (NR) ( Results Serum GSH content depleted significantly after a total dose of 4 Gy and 10 Gy of radiotherapy with a single dose of cisplatin, which was significantly lesser in NR than of CR patients. Similarly, Olive Tail Moment, the index of DNA damage, indicated significantly higher values in the fifth fraction of radiotherapy (5-RT) than in pretreatment. The DNA damage after 5-RT in the NR subgroup was significantly lower than that of CR. Conclusions Serum GSH analysis and tumor tissue SCGE assay found to be useful parameters for predicting chemoradioresponse prior to and also at an early stage of treatment of cervical cancers. |
| Databáze: | OpenAIRE |
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