A tissue culture infectious dose (TCID)-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on adherent cells
| Autor: | Fabio Hasler, Thomas M. Kündig, Agathe Duda, Pål Johansen |
|---|---|
| Přispěvatelé: | University of Zurich |
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Science (General)
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses Immunology 610 Medicine & health Genetics and Molecular Biology Enzyme-Linked Immunosorbent Assay Viral Plaque Assay Antibodies Viral Microbiology General Biochemistry Genetics and Molecular Biology Neutralization Virus Tissue culture Q1-390 Adherent cell Neutralization Tests Chlorocebus aethiops Protocol Medicine Animals Humans Vero Cells biology General Immunology and Microbiology business.industry SARS-CoV-2 Infectious dose General Neuroscience fungi 10177 Dermatology Clinic virus diseases COVID-19 Virology Antibodies Neutralizing Cell culture General Biochemistry biology.protein Cell-based Assays Antibody business |
| Zdroj: | STAR Protocols STAR Protocols, Vol 2, Iss 4, Pp 100824-(2021) |
| ISSN: | 2666-1667 |
| Popis: | For a cytopathic virus such as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), the neutralization capacity of serum from convalescent or vaccinated persons or of therapeutic antibodies can be tested on adherent cell cultures. Here, a simple and tissue culture infectious dose (TCID)-derived protocol for assessment of neutralization of SARS-CoV-2 is described. Compared to the often applied plaque-forming unit (PFU) assay, the working load is lower, and fewer manipulations of the infected cultures are required. Hence, the method is safer for the personnel. Graphical Abstract |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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