REG3γ-deficient mice have altered mucus distribution and increased mucosal inflammatory responses to the microbiota and enteric pathogens in the ileum

Autor: Jerry M. Wells, Linda M. P. Loonen, H.H. Kranenbarg-Stolte, P. van Baarlen, M.T.J. Jaklofsky, Jan Dekker, Marjolein Meijerink
Rok vydání: 2014
Předmět:
Salmonella
Interleukin-1beta
Pancreatitis-Associated Proteins
medicine.disease_cause
Mice
fluids and secretions
Deficient mouse
Immunology and Allergy
bacterial aggregation
Intestinal Mucosa
Dietary calcium
Mice
Knockout
dietary calcium
Microbiota
regiii-gamma
STAT1 Transcription Factor
medicine.anatomical_structure
Interferon Regulatory Factors
bowel-disease
Signal Transduction
STAT3 Transcription Factor
pancreatitis-associated protein
salmonella
Immunology
Ileum
Biology
digestive system
Microbiology
expression
medicine
Animals
Distribution (pharmacology)
Host-Microbe Interactomics
Inflammation
Gene Expression Profiling
Proteins
Lectin
Listeria monocytogenes
Mucus
Immunity
Innate
infection
Toll-Like Receptor 3
rats
Disease Models
Animal
Salmonella enteritidis
Myeloid Differentiation Factor 88
WIAS
biology.protein
lectin
Departement Dierwetenschappen
Interferons
Department of Animal Sciences
Zdroj: Mucosal Immunology, 7, 939-947
Mucosal Immunology 7 (2014)
ISSN: 1933-0219
DOI: 10.1038/mi.2013.109
Popis: REG3γ is considered to have a protective role against infection with Gram-positive bacteria due to its bactericidal activity, but evidence from in vivo studies is lacking. We generated a REG3γ(-/-) mouse, and investigated the effect of lack of REG3γ on intestinal mucus distribution, spatial compartmentalization of bacteria, and expression of innate immunity genes. Infection studies were also performed with Gram-positive and Gram-negative pathogens to investigate the antimicrobial role of REG3γ. REG3γ(-/-) mice display altered mucus distribution, increased bacterial contact with the epithelium, and elevated inflammatory markers in the ileum without histological evidence of pathology. Infection response pathway genes were differentially expressed in both Listeria monocytogenes and Salmonella enteritidis infected REG3γ(-/-) and wild-type (wt) mice. Higher amounts of myeloperoxidase and interleukin-22 transcripts were present in the ileal mucosa of REG3γ(-/-) than wt mice, but translocation to the organs was unaffected. We concluded that REG3γ has a protective role against mucosal infection with pathogenic Listeria and Salmonella in vivo. REG3γ is equally distributed throughout the mucus and its absence results in increased epithelial contact with the microbiota resulting in low-grade inflammation. REG3γ can bind to Gram-negative and Gram-positive bacteria and influence mucus distribution in the ileum, properties which may contribute to mucosal protection.
Databáze: OpenAIRE
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