Involvement of protein kinases in signalling for nitric oxide (NO) and tumour necrosis factor alpha (TNF) production by monocytes stimulated with colorectal DeTa cancer cells: the lack of evidence for the role of TNF in the regulation of NO production
| Autor: | Maciej Siedlar, Bozenna Mytar, Mariola Hyszko, Marek Zembala |
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| Rok vydání: | 1999 |
| Předmět: |
medicine.medical_specialty
Immunology Stimulation Endogeny Nitric Oxide Monocytes Nitric oxide chemistry.chemical_compound Internal medicine medicine Tumor Cells Cultured Immunology and Allergy Humans Enzyme Inhibitors Protein kinase C Kinase business.industry Tumor Necrosis Factor-alpha Carcinoma Endocrinology chemistry Cancer cell Cancer research Tumor necrosis factor alpha Signal transduction business Colorectal Neoplasms Protein Kinases Signal Transduction |
| Zdroj: | Immunology letters. 65(3) |
| ISSN: | 0165-2478 |
| Popis: | Kinases that are involved in NO and TNF production by human monocytes (MO) stimulated by colorectal cancer (DeTa) cells and effects of exogenous and endogenously synthesized TNF on NO induction were studied. The results based on the use of various inhibitors of protein kinases suggest that different signalling pathways operate in MO during induction of TNF and NO release after stimulation by DeTa cells. Stimulation of NO production required at least PTK, PKC and PKA, but only PTK and PKC were engaged in signal transduction for TNF production. Exogenous TNF and TNF produced by MO upon contact with DeTa cells was not sufficient for the induction or enhancement of NO synthesis in MO. The TNF synthesis was not influenced by neither exogenous nor endogenous NO produced by MO in the co-culture. Therefore, signal transduction pathways operating in MO during NO induction seem to be different from these engaged in TNF production, and both regulatory pathways probably operate in MO independently. |
| Databáze: | OpenAIRE |
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