The Drosophila formin Fhos is a primary mediator of sarcomeric thin-filament array assembly
| Autor: | Nagaraju Dhanyasi, Arkadi Shwartz, Eyal D. Schejter, Ben-Zion Shilo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Myofilament QH301-705.5 formin Science Actinin macromolecular substances Microfilament General Biochemistry Genetics and Molecular Biology Protein filament 03 medical and health sciences Biology (General) Actin General Immunology and Microbiology biology General Neuroscience muscle fiber General Medicine Anatomy Cell biology 030104 developmental biology Treadmilling muscle actin Formins biology.protein Medicine sarcomere Fhos/FHOD Drosophila Protein |
| Zdroj: | eLife, Vol 5 (2016) |
| Popis: | Actin-based thin filament arrays constitute a fundamental core component of muscle sarcomeres. We have used formation of the Drosophila indirect flight musculature for studying the assembly and maturation of thin-filament arrays in a skeletal muscle model system. Employing GFP-tagged actin monomer incorporation, we identify several distinct phases in the dynamic construction of thin-filament arrays. This sequence includes assembly of nascent arrays after an initial period of intensive microfilament synthesis, followed by array elongation, primarily from filament pointed-ends, radial growth of the arrays via recruitment of peripheral filaments and continuous barbed-end turnover. Using genetic approaches we have identified Fhos, the single Drosophila homolog of the FHOD sub-family of formins, as a primary and versatile mediator of IFM thin-filament organization. Localization of Fhos to the barbed-ends of the arrays, achieved via a novel N-terminal domain, appears to be a critical aspect of its sarcomeric roles. |
| Databáze: | OpenAIRE |
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