Different strategies for cyclodextrin production: Ultrafiltration systems, CGTase immobilization and use of a complexing agent
| Autor: | Vanderson Carvalho Fenelon, Luciana Numata Koga, Graciette Matioli, Aline Satomi Noce, Camila Sampaio Mangolim, Juliana Harumi Miyoshi |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Polymers and Plastics Ultrafiltration Bacillus Curdlan Ferric Compounds 01 natural sciences Continuous production Phosphates Substrate Specificity chemistry.chemical_compound 010608 biotechnology Materials Chemistry Chelation Glycyrrhizin chemistry.chemical_classification Cyclodextrins Chromatography Cyclodextrin biology 010405 organic chemistry Chemistry Organic Chemistry Enzymes Immobilized biology.organism_classification 0104 chemical sciences Glucosyltransferases Bacillus firmus Selectivity |
| Zdroj: | Carbohydrate Polymers. 192:19-27 |
| ISSN: | 0144-8617 |
| DOI: | 10.1016/j.carbpol.2018.03.035 |
| Popis: | The study comparatively evaluated diverse strategic models of cyclodextrin (CD) production by the CGTase of Bacillus firmus strain 37: continuous production and repetitive batches in ultrafiltration systems; immobilization of CGTase on curdlan and vegetable sponge natural supports; the use of the glycyrrhizin complexing agent to modulate CGTase selectivity in favor of γ-CD production. All strategies had in common the possibility of separation of CGTase from its inhibitory products and its reuse. In the continuous production model, at 48 h of assay, the highest productivity and selectivity for β-CD were obtained, 1.47 mmol/L/h and 92.8%, respectively. Glycyrrhizin was able to modulate the production of γ-CD with selectivity of 61.2% for 30-h batches. The comparative evaluation of the different strategic models for obtaining CDs showed particularities that should be considered, and most of the models studied returned satisfactory yields as well as excellent selectivity. |
| Databáze: | OpenAIRE |
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