Biosynthesis of thiamine by Phycomyces blakesleeanus
| Autor: | M. R. Houston, Ilda McVeigh |
|---|---|
| Rok vydání: | 1973 |
| Předmět: |
Immunology
Applied Microbiology and Biotechnology Microbiology Phosphates chemistry.chemical_compound Broth medium Biosynthesis Genetics Carbon Radioisotopes Thiamine Molecular Biology Chromatography biology Fungi General Medicine biology.organism_classification Chromatography Ion Exchange Culture Media Diphosphates Thiazoles Pyrimidines Biochemistry chemistry Solvents Autoradiography Phycomyces blakesleeanus |
| Zdroj: | Canadian journal of microbiology. 19(8) |
| ISSN: | 0008-4166 |
| Popis: | Phycomyces blakesleeanus was grown in a minimal glucose–asparagine broth medium supplemented with either 2-methyl-4-amino-5-hydroxymethylpyrimidine-4,7-14C or 4-methyl-5-(2-hydroxyethyl)-thiazole-2-14C and the corresponding non-labeled moiety of thiamine. Extracts of mycelia and of culture filtrates were tested for the presence of intermediates formed in the biosynthesis of thiamine by P. blakesleeanus. By combinations of ion-exchange and thin-layer chromatography, autoradiography, and bioautography, the following compounds were identified: 4-methyl-5-(2-phosphorylethyl)thiazole, 2-methyl-4-amino-5-phosphorylmethylpyrimidine, 2-methyl-4-amino-5-pyrophosphorylmethylpyrimidine, and thiamine monophosphate. |
| Databáze: | OpenAIRE |
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