Superior Protective Immunity against Murine Listeriosis by Combined Vaccination with CpG DNA and RecombinantSalmonella entericaSerovar Typhimurium
| Autor: | Elisabeth Roider, Holger Rüssmann, Jürgen Heesemann, Stefan Jellbauer, Klaus Panthel, Brigitte Köhn, Miriam Partilla, Christina Berchtold, Carole Bourquin, Stefan Endres |
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| Rok vydání: | 2009 |
| Předmět: |
Salmonella typhimurium
Salmonella Injections Subcutaneous Genetic Vectors Immunology Administration Oral CD8-Positive T-Lymphocytes medicine.disease_cause Microbiology Interleukin-7 Receptor alpha Subunit Mice Adjuvants Immunologic Bacterial Proteins T-Lymphocyte Subsets Immunity medicine Animals Cytotoxic T cell Listeriosis L-Selectin Mice Inbred BALB C Vaccines Synthetic biology biology.organism_classification Listeria monocytogenes Survival Analysis Virology Bacterial vaccine Vaccination Infectious Diseases Oligodeoxyribonucleotides CpG site Immunization Salmonella enterica Microbial Immunity and Vaccines Bacterial Vaccines Female Parasitology Spleen |
| Zdroj: | Infection and Immunity. 77:5501-5508 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.00700-09 |
| Popis: | Preexisting antivector immunity can severely compromise the ability ofSalmonella entericaserovar Typhimurium live vaccines to induce protective CD8 T-cell frequencies after type III secretion system-mediated heterologous protein translocation in orally immunized mice. To circumvent this problem, we injected CpG DNA admixed to the immunodominant p60217-225peptide fromListeria monocytogenessubcutaneously into BALB/c mice and coadministered a p60-translocatingSalmonellastrain by the orogastric route. The distribution of tetramer-positive p60217-225-specific effector and memory CD8 T cells was analyzed by costaining of lymphocytes with CD62L and CD127. In contrast to the single oral application of recombinantSalmonellaor single immunization with CpG and p60, in the spleens from mice immunized with a combination of both vaccine types a significantly higher level of p60-specific CD8 T cells with a predominance of the effector memory T-cell subset was detected. In vivo protection studies revealed that this CD8 T-cell population conferred sterile protective immunity against a lethal infection withL. monocytogenes. However, p60-specific central memory CD8 T cells induced by single vaccination with CpG and p60 were not able confer effective protection against rapidly replicating intracellularListeria. In conclusion, we provide compelling evidence that the combination ofSalmonellatype III-mediated antigen delivery and CpG immunization is an attractive novel vaccination strategy to modulate CD8 differentiation patterns toward distinct antigen-specific T-cell subsets with favorable protective capacities. |
| Databáze: | OpenAIRE |
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