Microcystin-LR-Triggered Neuronal Toxicity in Whitefish Does Not Involve MiR124-3p
Autor: | Maciej Woźny, Alicja Łakomiak, Maciej Florczyk, Ewa Jakimiuk, Paweł Brzuzan |
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Rok vydání: | 2018 |
Předmět: |
0301 basic medicine
Microcystins Biology Transfection Toxicology Animals Genetically Modified Circulating microRNA 03 medical and health sciences 0302 clinical medicine Glial Fibrillary Acidic Protein microRNA Neurotoxicity medicine Animals Humans Gliosis Enzyme Inhibitors Phylogeny Messenger RNA Reporter gene Glial fibrillary acidic protein General Neuroscience Hepatotoxin Brain medicine.disease Up-Regulation Cell biology Disease Models Animal MicroRNAs HEK293 Cells 030104 developmental biology medicine.anatomical_structure nervous system biology.protein Original Article Marine Toxins Neurotoxicity Syndromes medicine.symptom Salmonidae 030217 neurology & neurosurgery Astrocyte |
Zdroj: | Neurotoxicity Research |
ISSN: | 1476-3524 1029-8428 |
Popis: | Microcystin-LR (MC-LR) is a potent hepatotoxin that has also been pointed out of causing neurotoxicity, but the exact mechanisms of action still remain ambiguous and need to be elucidated. Data from studies on mammals show that pathology of astrocyte cells points to perturbations of microRNA signaling. Glial fibrillary acidic protein (GFAP), a neuronal cell/astrocyte-specific protein, and a microRNA-124-3p (MiR124-3p) are among putative triggers and regulators of neuronal cell/astrocyte reactivity. In the present study on whitefish (Coregonus lavaretus), we found that gfap mRNA contains a putative target site for MIR124-3p, to potentially affect its expression changes. qPCR expression study of gfap:MiR124-3p pair in the midbrain of juvenile whitefish, during 28 days of exposure to a repeated subacute dose of MC-LR (100 μg kg−1 body mass), showed marginally significant up-regulation of gfap only on the 7th day of exposure period which suggests neuronal toxicity. During the whole exposure period, neither midbrain nor blood plasma levels of MiR124-3p were changed. Furthermore, double luciferase gene reporter assay confirmed the lack of MiR124-3p involvement in mediating control over gfap mRNA expression. These data show that, although MC-LR may trigger neuronal toxicity in whitefish, this does not involve MiR124-3p in response to the treatment. Electronic supplementary material The online version of this article (10.1007/s12640-018-9920-4) contains supplementary material, which is available to authorized users. |
Databáze: | OpenAIRE |
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